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Given this, genetic tools are needed to robustly identify\n                    <jats:italic>Pocillopora<\/jats:italic>\n                    individuals to the species level. Quick and accurate identification approaches for\n                    <jats:italic>Pocillopora<\/jats:italic>\n                    species are critical to estimating biodiversity patterns under current and future environmental challenges. In recent years, the mitochondrial open reading frame (mtORF) and a histone region (PocHistone) have been validated using genome\u2010wide data to become the most widely used species\u2010level markers for\n                    <jats:italic>Pocillopora<\/jats:italic>\n                    . However, Sanger sequencing of a large number of samples can be prohibitively expensive and sequencing facilities are not always readily available. Therefore, we present restriction fragment length polymorphism (RFLP) digests here that identify the six species of\n                    <jats:italic>Pocillopora<\/jats:italic>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. acuta<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:italic>P.<\/jats:italic>\n                    cf.\n                    <jats:italic>effusa<\/jats:italic>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. grandis<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. meandrina<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:italic>P. tuahiniensis<\/jats:italic>\n                    , and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. verrucosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) found in French Polynesia, without sequencing. In uninformed validation tests (in silico and in\u00a0vitro), our protocol identified each\n                    <jats:italic>Pocillopora<\/jats:italic>\n                    species with 100% accuracy. Given their cost\u2010effective, rapid nature, the tailoring of additional RFLP digest protocols to identify cryptic coral species in reef regions around the world will support foundational reef science, conservation and restoration initiatives.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74409","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:21:44Z","timestamp":1790929304000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Genetic Method for Distinguishing Cryptic\n                    <i>Pocillopora<\/i>\n                    Species in French Polynesia Without Sequencing"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2843-6614","authenticated-orcid":false,"given":"Francesca M.","family":"Cohn","sequence":"first","affiliation":[{"name":"Department of Environmental Science, Policy, and Management University of California  Berkeley California USA"},{"name":"Department of Developmental Biology Stanford University  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0520-9324","authenticated-orcid":false,"given":"Erika C.","family":"Johnston","sequence":"additional","affiliation":[{"name":"Hawaii Institute of Marine Biology University of Hawaii at Manoa  Kaneohe Hawaii USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0348-3453","authenticated-orcid":false,"given":"Scott C.","family":"Burgess","sequence":"additional","affiliation":[{"name":"Department of Biological Science Florida State University  Tallahassee Florida USA"},{"name":"Department of Biology Norwegian University of Science and Technology  Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3107-0808","authenticated-orcid":false,"given":"Jordan A.","family":"Sims","sequence":"additional","affiliation":[{"name":"Department of Environmental Science, Policy, and Management University of California  Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1991-3672","authenticated-orcid":false,"given":"Krithika","family":"Layagala","sequence":"additional","affiliation":[{"name":"Department of Environmental Science, Policy, and Management University of California  Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5495-1868","authenticated-orcid":false,"given":"Pierrick","family":"Harnay","sequence":"additional","affiliation":[{"name":"Leon H. Charney School of Marine Sciences University of Haifa  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2322-3269","authenticated-orcid":false,"given":"Hollie M.","family":"Putnam","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences College of the Environment and Life Sciences, University of Rhode Island  Kingston Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0137-5042","authenticated-orcid":false,"given":"Adrienne M. S.","family":"Correa","sequence":"additional","affiliation":[{"name":"Department of Environmental Science, Policy, and Management University of California  Berkeley California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecy.3324"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecy.70079"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/eva.13762"},{"key":"e_1_2_12_5_1","doi-asserted-by":"crossref","unstructured":"Cohn F. 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