{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T10:50:21Z","timestamp":1791802221624,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The complete chloroplast genome of\n                    <jats:italic>Dioscorea hypoglauca<\/jats:italic>\n                    \u2014a medicinal plant native to southern China\u2014was assembled and characterized. The genome is circular, 153,219\u2009bp in length, and follows the typical quadripartite organization: a large single\u2010copy (LSC) region of 83,692\u2009bp, a small single\u2010copy (SSC) region of 18,813\u2009bp, and two inverted repeat (IR) regions, each 25,357\u2009bp. In total, 129 genes were annotated, including 84 protein\u2010coding genes, 37 tRNA genes, and eight rRNA genes. Thirty\u2010five simple sequence repeats (SSRs) were detected; most were mononucleotide A\/T repeats. A comparison with 22 other\n                    <jats:italic>Dioscorea<\/jats:italic>\n                    species showed that IR boundaries vary considerably across the genus. One notable finding is the boundary shift of the\n                    <jats:italic>ycf1<\/jats:italic>\n                    gene in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. hypoglauca<\/jats:italic>\n                    <\/jats:styled-content>\n                    , which has not been observed in other examined species. Nucleotide diversity (Pi) analysis revealed that intergenic spacers\u2010especially\n                    <jats:italic>rpl22\u2010rps19<\/jats:italic>\n                    (Pi\u2009=\u20090.11204)\u2010are highly variable, whereas tRNA genes are extremely conserved. Codon usage analysis indicated a strong bias toward A\/T\u2010ending codons. Phylogenetic reconstruction based on complete plastomes (using maximum likelihood and Bayesian inference) placed\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. hypoglauca<\/jats:italic>\n                    <\/jats:styled-content>\n                    as a close relative of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. aspersa<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:italic>D. pyrifolia<\/jats:italic>\n                    . Overall, these results clarify the systematic position of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. hypoglauca<\/jats:italic>\n                    <\/jats:styled-content>\n                    , illustrate the dynamic evolution of IR regions within\n                    <jats:italic>Dioscorea<\/jats:italic>\n                    , and provide a genomic resource for future evolutionary and population genetic studies.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74420","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T02:09:37Z","timestamp":1791770977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Complete Chloroplast Genome of\n                    <i>Dioscorea hypoglauca<\/i>\n                    : Comparative Genomics and Phylogenetic Insights"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1166-2725","authenticated-orcid":false,"given":"Gang","family":"Li","sequence":"first","affiliation":[{"name":"Bozhou Vocational and Technical College  Bozhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daofeng","family":"Gong","sequence":"additional","affiliation":[{"name":"Bozhou Vocational and Technical College  Bozhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Bozhou Vocational and Technical College  Bozhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"Bozhou Vocational and Technical College  Bozhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Chen","sequence":"additional","affiliation":[{"name":"Bozhou Vocational and Technical 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