{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:51:21Z","timestamp":1791431481097,"version":"4.3.3"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004595","name":"Universiti Sains Malaysia","doi-asserted-by":"publisher","award":["R501\u2010LR\u2010RND003\u20100000001594\u20100000"],"award-info":[{"award-number":["R501\u2010LR\u2010RND003\u20100000001594\u20100000"]}],"id":[{"id":"10.13039\/501100004595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The tropical barn owl (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Tyto javanica javanica<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) is a major biocontrol agent against rodents in paddy fields. While raptors are frequently monitored for zoonotic diseases, they may also facilitate beneficial microbial redistribution. This study evaluated bacterial communities in barn owl regurgitated pellets to assess their role as environmental biosensors and vectors for microbe dispersal. We collected 90 fresh pellets from three paddy field locations during the growing season. Samples underwent bacterial enumeration and metagenomic sequencing of the 16S rRNA V3\u2010V4 domains via the Illumina MiSeq platform. Results revealed significant spatial variation in bacterial communities across locations (PERMANOVA,\n                    <jats:italic>F<\/jats:italic>\n                    (2, 87)\u2009=\u20093.14,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Firmicutes (28.1%\u201363.2%) and Proteobacteria (17.7%\u201345.6%) dominated all sites. Notably,\n                    <jats:italic>Sporosarcina<\/jats:italic>\n                    sp., known for eco\u2010friendly biocementation and soil enhancement, was highly abundant across all locations (up to 40.6%). The high abundance of\n                    <jats:italic>Escherichia<\/jats:italic>\n                    sp. at the AV site (32.1%) highlighted the pellet's ability to capture transient environmental and prey\u2010derived coliforms. Ultimately, these pellets act as critical environmental proxies capturing localized microbiota. This supports the perspective that barn owls contribute significantly more to microbial ecological redistribution than to disease spread, offering valuable agricultural ecological services.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74429","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:25:23Z","timestamp":1790223923000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metagenomic Profiling of Barn Owl (\n                    <scp>\n                      <i>Tyto javanica javanica<\/i>\n                    <\/scp>\n                    ) Regurgitated Pellets Reveals Spatially Structured Environmental Microbiota in Paddy Agroecosystems"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6582-931X","authenticated-orcid":false,"given":"Kamarul Zaman","family":"Zarkasi","sequence":"first","affiliation":[{"name":"Microbial Ecology and Bacteriology Laboratory, School of Biological Sciences Universiti Sains Malaysia  Minden Pulau Pinang Malaysia"},{"name":"Barn Owl and Rodent Research Group (BORG), Universiti Sains Malaysia  Minden Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Hasif Ahmad","family":"Kamal","sequence":"additional","affiliation":[{"name":"Microbial Ecology and Bacteriology Laboratory, School of Biological Sciences Universiti Sains Malaysia  Minden Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Deanni","family":"Ronidean","sequence":"additional","affiliation":[{"name":"Microbial Ecology and Bacteriology Laboratory, School of Biological Sciences Universiti Sains Malaysia  Minden Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Syafiq Mohd","family":"Zaludin","sequence":"additional","affiliation":[{"name":"Barn Owl and Rodent Research Group (BORG), Universiti Sains Malaysia  Minden Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Feizal","family":"Daud","sequence":"additional","affiliation":[{"name":"Faculty of Plantation and Agrotechnology Universiti Teknologi MARA Malacca Branch, Jasin Campus  Merlimau Malacca Malaysia"},{"name":"Field Crops Production and Crop Quality Product Research Group, Faculty of Plantation and Agrotechnology Universiti Teknologi MARA Malacca Branch, Jasin Campus  Merlimau Malacca Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susiana","family":"Purwantisari","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science and Mathematics Universitas Diponegoro  Semarang Central Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir Hamzah Ahmad","family":"Ghazali","sequence":"additional","affiliation":[{"name":"Microbial Ecology and Bacteriology Laboratory, School of Biological Sciences Universiti Sains Malaysia  Minden Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasber","family":"Salim","sequence":"additional","affiliation":[{"name":"Barn Owl and Rodent Research Group (BORG), Universiti Sains Malaysia  Minden Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.202000695"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.21161\/mjm.211208"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2015.10.007"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.101612"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01830"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2021.642345"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.6299"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.1539"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14212"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11010022"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.791287"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2013.06.001"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/birds2010003"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010024\u201004163\u2010z"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jav.01788"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02258"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.21161\/mjm.190564"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/14777606221085988"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10965\u2010023\u201003630\u20109"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179945"},{"issue":"3","key":"e_1_2_12_22_1","first-page":"897","article-title":"Microbial 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Z.","year":"2019","journal-title":"Jordan Journal of Biological Sciences"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.21161\/mjm.230018"}],"container-title":["Ecology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.74429","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ece3.74429","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.74429","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:04:27Z","timestamp":1791428667000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ece3.74429"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":41,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/ece3.74429"],"URL":"https:\/\/doi.org\/10.1002\/ece3.74429","archive":["Portico"],"relation":{},"ISSN":["2045-7758","2045-7758"],"issn-type":[{"value":"2045-7758","type":"print"},{"value":"2045-7758","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e74429"}}