{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:38:51Z","timestamp":1792600731688,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dothistroma needle blight (DNB), caused by\n                    <jats:italic>Dothistroma septosporum<\/jats:italic>\n                    , is a globally important disease of pines, yet its ecological role within native host systems remains incompletely understood. We combined species\u2010specific quantitative PCR (qPCR) with ITS2 metabarcoding to investigate the occurrence of\n                    <jats:italic>D. septosporum<\/jats:italic>\n                    and associated needle mycobiomes of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pinus sylvestris<\/jats:italic>\n                    <\/jats:styled-content>\n                    in natural stands in Slovakia with a pooled non\u2010natural positive\u2010reference group included for comparison.\n                    <jats:italic>D. septosporum<\/jats:italic>\n                    was detected in the Vern\u00e1r natural stand (11 of 16 samples), where visible DNB symptoms were also observed, and in all samples of the non\u2010natural positive\u2010reference group (15 of 15 samples). The pathogen was not detected in the other three sampled natural stands. Fungal community analyzes revealed significant differences in community composition among sample groups, while alpha diversity patterns showed comparatively minor variation. The most abundant genus across all sites was\n                    <jats:italic>Lophodermium<\/jats:italic>\n                    , whereas\n                    <jats:italic>Dothistroma<\/jats:italic>\n                    was highly abundant only in the non\u2010natural positive\u2010reference group. Co\u2010occurrence network analysis indicated that fungal communities were dominated by positive associations; however, patterns involving\n                    <jats:italic>Dothistroma<\/jats:italic>\n                    primarily reflected the positive\u2010reference group, where the pathogen was highly abundant. Our results suggest that\n                    <jats:italic>D. septosporum<\/jats:italic>\n                    is not a consistently detectable component of needle mycobiomes in native\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. sylvestris<\/jats:italic>\n                    <\/jats:styled-content>\n                    stands in Slovakia and that its high abundance in the non\u2010natural positive\u2010reference group was associated with a distinct fungal community composition.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74481","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T05:20:49Z","timestamp":1792214449000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fungal Needle Communities and\n                    <i>Dothistroma septosporum<\/i>\n                    Occurrence in\n                    <i>Pinus sylvestris<\/i>\n                    Stands in Slovakia"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7885-6066","authenticated-orcid":false,"given":"Renata","family":"Artimov\u00e1","sequence":"first","affiliation":[{"name":"Department of Plant Pathology and Mycology, Institute of Forest Ecology Slovak Academy of Sciences  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5407-5233","authenticated-orcid":false,"given":"Katar\u00edna","family":"Adam\u010d\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Mycology, Institute of Forest Ecology Slovak Academy of Sciences  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2430-5530","authenticated-orcid":false,"given":"Juraj","family":"Medo","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Faculty of Biotechnology and Food Science Slovak University of Agriculture in Nitra  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0521-6778","authenticated-orcid":false,"given":"Marek","family":"Kobza","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Mycology, Institute of Forest Ecology Slovak Academy of Sciences  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5082-8709","authenticated-orcid":false,"given":"Em\u00edlia","family":"Ondru\u0161kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Mycology, Institute of Forest Ecology Slovak Academy of Sciences  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8746-389X","authenticated-orcid":false,"given":"Zuzana","family":"J\u00e1no\u0161\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Mycology, Institute of Forest Ecology Slovak Academy of Sciences  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.15287\/afr.2023.2648"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2017.12.001"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9081757"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755\u20100998.2008.02142.x"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.1200"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.13383"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.12947"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010933404324"},{"key":"e_1_2_12_10_1","unstructured":"Brown A. 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