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These temporary symbiotic associations have been important in driving the evolutionary development of this taxon. The phoretic mite genus\n                    <jats:italic>Poecilochirus<\/jats:italic>\n                    , which is considered obligately associated with burying beetles, is of great value in forensic entomology. However, due to the morphological conservatism and the difficulty of obtaining adult specimens, the taxonomy of this genus has long been based on deutonymphal characters, and the true species diversity is far from resolved. Here we used next\u2010generation sequencing to determine, for the first time, the complete mitochondrial genomes of\n                    <jats:italic>P. austroasiaticus<\/jats:italic>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. subterraneus<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and two distinct lineages of\n                    <jats:italic>P. carabi<\/jats:italic>\n                    (BJ1 and BJ2). All the sequenced mitogenomes contain 37 genes with a gene order identical to that of the ancestral arthropod arrangement and they all show pronounced AT bias and negative GC skew. All 13 protein\u2010coding genes are under strong purifying selection (\n                    <jats:italic>Ka\/Ks<\/jats:italic>\n                    &lt;\u20091). Sliding window analysis identifies\n                    <jats:italic>nad2<\/jats:italic>\n                    and\n                    <jats:italic>nad5<\/jats:italic>\n                    as nucleotide diversity hotspots, which could serve as high\u2010resolution markers for future species delimitation. Phylogenetic reconstruction tentatively recovered\n                    <jats:italic>Poecilochirus<\/jats:italic>\n                    as paraphyletic with\n                    <jats:italic>Parasitus<\/jats:italic>\n                    nested within it (ML\u2009=\u200944%, BI\u2009=\u20090.982), while Parasitinae and Pergamasinae are not reciprocally monophyletic. The\n                    <jats:italic>cox1<\/jats:italic>\n                    genetic distance between\n                    <jats:italic>P. carabi<\/jats:italic>\n                    BJ1 and BJ2 reached 0.409, with both mPTP and ASAP analyses of the mitogenomic dataset partitioning them into separate candidate molecular operational taxonomic units. Bayesian divergence time estimation revealed that the two lineages diverged in the Eocene about 52.4 million years ago (Ma), before the split between the morphologically recognized species\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. subterraneus<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:italic>P. davydovae<\/jats:italic>\n                    (39.0\u2009Ma). Our results give strong molecular evidence for cryptic speciation within the\n                    <jats:italic>P. carabi<\/jats:italic>\n                    complex and provide a solid genomic foundation for the future systematic and taxonomic research in the Parasitidae.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74501","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T01:55:36Z","timestamp":1791770136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mitogenomic Insights Into the Ancient Split and Cryptic Diversity of the\n                    <i>Poecilochirus carabi<\/i>\n                    Complex (Acari: Mesostigmata)"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9512-4368","authenticated-orcid":false,"given":"Lang","family":"Liang","sequence":"first","affiliation":[{"name":"The Provincial Key Laboratory for Plant Pest Management of Mountainous Region, Scientific Observing and Experimental Station of Crop Pest in Guiyang, Institute of Entomology, Ministry of Agricultural and Rural Affairs Guizhou University  Guiyang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Yue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Guizhou Civil Aviation Vocational College  Anshun P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu\u2010Die","family":"He","sequence":"additional","affiliation":[{"name":"The Provincial Key Laboratory for Plant Pest Management of Mountainous Region, Scientific Observing and Experimental Station of Crop Pest in Guiyang, Institute of Entomology, Ministry of Agricultural and Rural Affairs Guizhou University  Guiyang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian\u2010Ci","family":"Yi","sequence":"additional","affiliation":[{"name":"The Provincial Key Laboratory for Plant Pest Management of Mountainous Region, Scientific Observing and Experimental Station of Crop Pest in Guiyang, Institute of Entomology, Ministry of Agricultural and Rural Affairs Guizhou University  Guiyang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dao\u2010Chao","family":"Jin","sequence":"additional","affiliation":[{"name":"The Provincial Key Laboratory for Plant Pest Management of Mountainous Region, Scientific Observing and Experimental Station of Crop Pest in Guiyang, Institute of Entomology, Ministry of Agricultural and Rural Affairs Guizhou University  Guiyang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAF.0000000000001067"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414\u2010006\u20100086\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz255"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/24750263.2023.2288847"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.5958\/0974\u20108172.2019.00041.5"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010011\u20102767\u20108"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/d14110948"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.24349\/acarologia\/20194328"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.5194\/egusphere\u20102025\u20106383"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.70203"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16248"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz833"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10493\u2010009\u20109247\u20101"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scijus.2020.11.003"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2006.03.006"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz238"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0288991"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.24349\/acarologia\/20184280"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13061074"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.5962\/p.12620"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx025"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm573"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026360"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095\u20108137.2021.418"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/zsc.12369"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/01647954.2024.2389343"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.11158\/saa.28.3.15"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10493\u2010026\u201001183\u20102"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.11158\/saa.31.2.10"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.11158\/saa.29.6.9"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw413"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/01647954.2025.2480676"},{"issue":"2","key":"e_1_2_11_39_1","first-page":"79","article-title":"Replacement Name for Cornigamasus lunaroides Ma, 1986 and Studies of the Genus Poecilochirus in Northern China (Acari: Gamasina: Parasitidae)","volume":"14","author":"Ma L.\u2010M.","year":"2005","journal-title":"Acta Arachnologica Sinica"},{"issue":"5","key":"e_1_2_11_40_1","first-page":"515","article-title":"Mites (Acarina) Associated With Burying and Carrion Beetles (Coleoptera, Silphidae) and Description of Poecilochirus mrciaki sp. 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