{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T20:42:51Z","timestamp":1777408971888,"version":"3.51.4"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["esajournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nitrogen (N)\u2010fixation is a crucial source of reactive N in terrestrial environments. The nitrogenase enzyme (Nase), responsible for this conversion, has three isoforms: molybdenum (Mo)\u2010, vanadium (V)\u2010, and iron (Fe)\u2010Nases. The Mo\u2010Nase is found in all N\u2010fixers, but the distribution pattern and drivers of V\u2010 and Fe\u2010Nases remain poorly understood. Carbon and micronutrients (Mo and V) might be key contributors to N\u2010fixation by affecting the metabolic demand for N and participating in Nase synthesis, respectively. Here, we investigated the presence of V\u2010Nase genes in a tropical cyanolichen and explored the impact of micronutrient supply and elevated carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ) concentrations on N\u2010fixation. The experiment revealed a significant influence of V and CO\n                    <jats:sub>2<\/jats:sub>\n                    concentrations on heterocyst investment. V\u2010Nase genes were also detected in tropical lichen specimens, suggesting that tropical species might have both Mo\u2010 and V\u2010Nases. Our study suggests that V\u2010Nase is prevalent beyond cold environments, by reporting its presence in the warm tropics and demonstrating a significant response of N\u2010fixing cells to V addition. We emphasize the importance of further research on N\u2010fixation in the tropics, particularly on the role of different Nase isoforms.\n                  <\/jats:p>","DOI":"10.1002\/ecy.70367","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T12:42:36Z","timestamp":1775047356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Coming out of the cold: Vanadium is an important player in N\u2010fixation in warm tropical environments"],"prefix":"10.1002","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6430-9080","authenticated-orcid":false,"given":"Ana","family":"F\u00e1varo","sequence":"first","affiliation":[{"name":"Departamento de Ecologia e Conserva\u00e7\u00e3o Universidade Federal de Lavras  Lavras Minas Gerais Brazil"},{"name":"Department of Ecology, Evolution and Behavior University of Minnesota\u2010Twin Cities  Saint Paul Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela Dal","family":"Forno","sequence":"additional","affiliation":[{"name":"Botanical Research Institute of Texas, Fort Worth Botanic Garden  Fort Worth Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6713-9328","authenticated-orcid":false,"given":"Daniel","family":"Stanton","sequence":"additional","affiliation":[{"name":"Department of Ecology, Evolution and Behavior University of Minnesota\u2010Twin Cities  Saint Paul Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara Cec\u00edlia Oliveira","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Universidade Federal de Lavras  Lavras Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via de Freitas","family":"Coelho","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Universidade Federal de Lavras  Lavras Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10533-020-00666-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0266467411000605"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10021-022-00804-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2013.05.006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12777"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1913314116"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c05279"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.5c12982"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14166"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14905"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo1486"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1127\/nova_hedwigia\/2021\/0644"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","unstructured":"F\u00e1varo A. 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