{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:59:14Z","timestamp":1790686754872,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302670"],"award-info":[{"award-number":["32302670"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["esajournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A fundamental challenge in microbiome research lies in elucidating the functional capacity of microbial communities through community membership and genomic data. As community structuring and emergent functional traits are determined by bacterial community metabolic networks, it is important to gain insights into the principles that govern bacteria\u2013bacteria interactions. Here, we applied an integrative framework linking individual strain\u2010level traits to community structuring in a simplified synthetic bacterial community (SSC8) that promotes the growth of ungrafted watermelon. By combining mono\u2010 and coculture assays with genome\u2010scale metabolic modeling and metabolomic profiling of spent media, we characterized directional interactions and resource dependencies among community members. Our findings show that positive interactions dominated the community network, accounting for 55% of all pairwise combinations, indicating a high prevalence of growth\u2010promoting effects among strains. Genome\u2010scale metabolic modeling showed that functional divergence among strains enhanced the potential for metabolic complementarity as phylogenetic distance increased. Integrating metabolic modeling with metabolomics further suggested that\n                    <jats:italic>Pseudomonas azotifigens<\/jats:italic>\n                    Q6 not only benefited from all other community members, but also exhibited mutualistic interactions with the other three strains, with metabolite exchange involving compounds such as L\u2010lysine and L\u2010cysteine.\n                    <jats:italic>Pseudomonas azotifigens<\/jats:italic>\n                    Q6 acted as an important driver of community composition by affecting the abundance of several other consortium members in vitro. These findings highlight the role of metabolic complementarity in driving community structuring by promoting selective persistence of specific strains. Our work provides mechanistic insights into microbial interaction networks in vitro and offers a conceptual foundation for the rational design of functionally robust and plant\u2010beneficial microbiomes.\n                  <\/jats:p>","DOI":"10.1002\/ecy.70474","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T04:25:36Z","timestamp":1788755136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Decoding microbial metabolic complementarity from individual traits to community structuring"],"prefix":"10.1002","volume":"107","author":[{"given":"Yizhu","family":"Qiao","sequence":"first","affiliation":[{"name":"Jiangsu Provincial Key Lab for Organic\u2010based Fertilizer Creation and Soil Health Manipulation Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Key Lab for Organic\u2010based Fertilizer Creation and Soil Health Manipulation Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Key Lab for Organic\u2010based Fertilizer Creation and Soil Health Manipulation Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qicheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Key Lab for Organic\u2010based Fertilizer Creation and Soil Health Manipulation Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Ruan","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Key Lab for Organic\u2010based Fertilizer Creation and Soil Health Manipulation Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qirong","family":"Shen","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Key Lab for Organic\u2010based Fertilizer Creation and Soil Health Manipulation Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1250-4073","authenticated-orcid":false,"given":"Ning","family":"Ling","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Key Lab for Organic\u2010based Fertilizer Creation and Soil Health Manipulation Nanjing Agricultural University  Nanjing China"},{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, Centre for Grassland Microbiome, College of Pastoral Agriculture Science and Technology Lanzhou University  Lanzhou 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