{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:00:29Z","timestamp":1790686829301,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DEB 1501483"],"award-info":[{"award-number":["DEB 1501483"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["esajournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Podocarps (Podocarpaceae) are one of the few lineages of conifers found in closed canopy tropical forests. They typically have highly restricted distributions that are associated with nutrient\u2010poor sites. Unlike other tree species, the presence of distinct nodule\u2010like structures allows their roots to be easily distinguished from those of co\u2010occurring taxa. In highly infertile ever\u2010wet lower montane forest at Fortuna, Panama, roots of\n                    <jats:italic>Podocarpus oleifolius<\/jats:italic>\n                    occur in three microsites: in the soil; lying on top of surface litter; as aerial roots that climb the trunks of neighboring trees. We examined how microsite affects\n                    <jats:italic>Podocarpus<\/jats:italic>\n                    root fungal community composition by sequencing the internal transcribed spacer (ITS) region, and by measuring root nutrient concentration and phosphatase enzyme activity. We also compared\n                    <jats:italic>Podocarpus<\/jats:italic>\n                    roots collected from the soil with those of two common co\u2010occurring angiosperm trees,\n                    <jats:italic>Graffenrieda bella<\/jats:italic>\n                    and\n                    <jats:italic>Weinmannia pinnata<\/jats:italic>\n                    . We found that root microsite accounted for 11% of the variation in root\u2010associated fungal communities and also affected root calcium, potassium, and manganese concentration. Root fungal diversity, phosphate concentration, and phosphatase activity were unchanged. Across all microsites, the fungal order Helotiales accounted for 50%\u201360% of ITS reads, while aerial roots had significantly higher abundance of Trechisporales. When comparing across species, Glomeromycota were only detected in very low numbers overall (1.8%\u20133.3% of reads in angiosperms), and were especially low in\n                    <jats:italic>Podocarpus<\/jats:italic>\n                    (0.14% of reads). In contrast,\n                    <jats:italic>Podocarpus<\/jats:italic>\n                    had a higher fraction of reads belonging to Leotiomycetes than\n                    <jats:italic>Graffenrieda<\/jats:italic>\n                    and\n                    <jats:italic>Weinmannia<\/jats:italic>\n                    . Our findings highlight how root\u2010associated fungal communities vary at the order level with microsite and host species in tropical montane forest. In continuously cool, low\u2010nutrient wet environments, where nutrients are rapidly leached from throughfall, or are mineralized slowly from decomposing organic matter, alternatives to arbuscular mycorrhizal fungi may be especially advantageous to plants. Biotrophic fungi in the Helotiales have been implicated in nutrient transfer in alpine and arctic environments, and together with Trechisporales, may be key unrecognized mutualistic partners in the tropics. Collectively, these taxa may explain the association of podocarps with low\u2010nutrient montane forest.\n                  <\/jats:p>","DOI":"10.1002\/ecy.70519","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:18:16Z","timestamp":1790320696000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct root\u2010associated fungal communities may account for the persistence of\n                    <i>Podocarpus<\/i>\n                    conifers in tropical forest"],"prefix":"10.1002","volume":"107","author":[{"given":"Astrid","family":"Ferrer","sequence":"first","affiliation":[{"name":"Department of Plant Biology University of Illinois  Urbana\u2010Champaign Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaine","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Plant Biology University of Illinois  Urbana\u2010Champaign Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Corrales","sequence":"additional","affiliation":[{"name":"Society for the Protection of Underground Networks  Dover Delaware USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6488-9895","authenticated-orcid":false,"given":"James W.","family":"Dalling","sequence":"additional","affiliation":[{"name":"Department of Plant Biology University of Illinois  Urbana\u2010Champaign Illinois USA"},{"name":"Smithsonian Tropical Research Institute  Panama City Republic of Panama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000454107"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ele.13129"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/2231385a0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01358347"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.18.060167.000543"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep39072"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2010.0485"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.5479\/si.0081024X.95.165"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2024.109363"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.5479\/si.0081024X.95.189"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","unstructured":"Chao A. andC. 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W.Dalling.2026.\u201cAncillary Data for Distinct Root Associated Fungal Communities May Account for the Persistence ofPodocarpusConifers in Tropical Forest.\u201dUniversity of Illinois Urbana\u2010Champaign.https:\/\/doi.org\/10.13012\/B2IDB-9955070_V1."},{"key":"e_1_2_9_24_1","first-page":"203","article-title":"The Role of Epiphytes in Rainfall Interception by a Lower Montane Tropical Forest in Panama","volume":"112","author":"G\u00f3mez G\u00f3nzalez D. C.","year":"2021","journal-title":"Smithsonian Contributions to Botany"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1139\/b03-153"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ele.13399"},{"key":"e_1_2_9_27_1","first-page":"747","volume-title":"Forest Environments in Tropical Life Zones: A Pilot Study","author":"Holdridge L. 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