{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:58:50Z","timestamp":1790290730924,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012021","name":"Iran University of Medical Sciences","doi-asserted-by":"publisher","award":["17152"],"award-info":[{"award-number":["17152"]}],"id":[{"id":"10.13039\/100012021","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Endocrino Diabet &amp;amp; Metabol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>In previous studies, the researchers observed an increase in insulin secretion in STZ\u2010treated diabetic rats following treatment with the hydroalcoholic extract of <jats:italic>Securigera securidaca<\/jats:italic> (HESS) seeds. This study focuses on the relationship between the antioxidant properties of HESS with changes in diabetic pancreatic tissue and the gene expression of factors that impact insulin secretion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this controlled experimental study, three varying doses of HESS were administered to three groups of diabetic rats induced by STZ. Oxidative stress indicators like total antioxidant capacity (TAC), total oxidant status (TOS) and malondialdehyde were assessed in both pancreatic and liver tissues. Pancreatic histology was studied post\u2010haematoxylin staining. Insulin and FGF21 levels in the blood were measured using the ELISA method. The expression of Nrf2 and FGF21 genes in the pancreas and liver, along with MafA and PDX\u20101 genes in the pancreas, was quantified using real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The administration of HESS in varying doses led to a dose\u2010dependent rise in blood insulin levels and a decrease in blood glucose levels and oxidative stress. By reducing oxidative stress, HESS treatment lowered the heightened levels of NRF2 and FGF21 in the liver and pancreas of diabetic rats, improving pancreatic tissue health. As oxidative stress decreased, the expression of MafA and PDX1 genes in the pancreas approached levels seen in healthy rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HESS elicits an increase in insulin secretion through the mitigation of oxidative stress and tissue damage, as well as the modulation of gene expression related to the insulin transcription factors PDX\u20101 and MafA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/edm2.515","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T05:18:56Z","timestamp":1725599936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enhanced Insulin Secretion Through Upregulation of Transcription Factors by Hydroalcoholic Extract of <i>Securigera securidaca<\/i> Seeds in Diabetic Animal Model"],"prefix":"10.1002","volume":"7","author":[{"given":"Maryam","family":"Hasani","sequence":"first","affiliation":[{"name":"Department of Biochemistry Medical School, Hamadan University of Medical Sciences  Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebrahim","family":"Abbasi\u2010Oshaghi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Medical School, Hamadan University of Medical Sciences  Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Khomari","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahar","family":"Kiani","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Mirzaei","sequence":"additional","affiliation":[{"name":"Department of Anatomical Sciences, School of Medicine Hamedan University of Medical Sciences  Hamedan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iraj","family":"Alipourfard","sequence":"additional","affiliation":[{"name":"University of Silesia  Katowice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iraj","family":"Khodadadi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Medical School, Hamadan University of Medical Sciences  Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heydar","family":"Tayebinia","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Medical School, Hamadan University of Medical Sciences  Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Babaei","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Science Bu\u2010Ali Sina University  Hamedan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahin","family":"Alizadeh\u2010Fanalou","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Urmia University of Medical Sciences  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6823-8638","authenticated-orcid":false,"given":"Elham","family":"Bahreini","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568009618666180430144441"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00008.2017"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112331"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7054272"},{"issue":"4","key":"e_1_2_11_6_1","first-page":"356","article-title":"The Streptozotocin\u2010Induced Diabetic Nude Mouse Model: Differences Between Animals From Different Sources","volume":"61","author":"Graham M. 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