{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T22:23:56Z","timestamp":1771885436902,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Endocrino Diabet &amp;amp; Metabol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>In childhood, growth hormone (GH) deficiency (GHD) diagnosis is based on auxological assessment and biochemical provocative tests, whose reliability remains disputed. Recently, several papers have been published on standardising the duration of some tests. The aim of our study was to analyse the possible length reduction of the L\u2010DOPA provocative test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively investigated the response of GH to L\u2010DOPA in 256 children, analysing 267 tests (some patients were retested over time for the persistence of severe auxopathy). We studied the same data considering GH peak threshold both at 8\u2009ng\/mL (Italian GHD cut\u2010off) and at 10\u2009ng\/mL (international cut\u2010off). Based on stimulation tests, patients were divided into two groups: GHD and no\u2010GHD short children. We described the results in the whole population and then clustering for gender and pubertal stage. We termed as index the test stopped at 90\u2009min.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The GH peak after L\u2010DOPA mostly occurred at 60\u2009min. The sensitivity of the index test was the highest, while the specificity was slightly higher using the 8\u2009ng\/mL threshold (specificity\u2009=\u20090.68; 95% CI 0.60\u20130.76) then using the 10\u2009ng\/mL threshold (specificity\u2009=\u20090.56; 95% CI 0.47\u20130.65) at 90\u2009min. The two ROC curves showed moderate performance of the test at 90\u2009min. While the negative predictive value was 100% in both tests, the positive predictive value was slightly better with 10\u2009ng\/mL cut\u2010off. Considering the two groups established by GHD definition and placing a GH threshold at 10\u2009ng\/mL, stopping L\u2010DOPA test time at 90\u2009min would have changed the test result and subsequentially patient's classification in 3\/267 of the analysed tests (1.1%), while with the Italian GH threshold value at 8\u2009ng\/mL in 7\/267 of the tests (2.6%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our research shows that omitting 120\u2010min time reduces L\u2010DOPA test specificity, especially with GHD cut\u2010off at 10\u2009ng\/mL.<\/jats:p><\/jats:sec>","DOI":"10.1002\/edm2.70000","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T08:38:40Z","timestamp":1725007120000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["L\u2010<scp>DOPA<\/scp> Test in the Diagnosis of Childhood Short Stature: Evaluation of Growth Hormone Peaks Over Time"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0311-8824","authenticated-orcid":false,"given":"Barbara","family":"Castelli","sequence":"first","affiliation":[{"name":"Health Sciences Department University of Florence  Florence Italy"},{"name":"Meyer Children's Hospital IRCCS  Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"De\u00a0Santis","sequence":"additional","affiliation":[{"name":"Meyer Children's Hospital IRCCS  Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Carrera","sequence":"additional","affiliation":[{"name":"Health Sciences Department University of Florence  Florence Italy"},{"name":"Meyer Children's Hospital IRCCS  Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco\u00a0Andrea","family":"Malanima","sequence":"additional","affiliation":[{"name":"Clinical Trial Center Careggi University Hospital  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