{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:41:15Z","timestamp":1786977675349,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T00:00:00Z","timestamp":1744588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Endocrino Diabet &amp;amp; Metabol"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Diabetes mellitus (DM) is a serious health condition affecting people worldwide with a high prevalence rate. DM is classified according to its aetiology into type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM); several risk factors contribute to the development of T2DM, such as age, genetic predisposition and obesity. Metformin is a proven drug of choice, and it is used as a first\u2010line treatment for patients with T2DM to improve their glycaemic levels. Vitamin B12 is an important water\u2010soluble vitamin, and its deficiency can lead to megaloblastic anaemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>A Cross\u2010sectional retrospective study was conducted on patients with T2DM in a Sedi Hussein diabetic polyclinic in Benghazi, Libya. The total sample was 381 patients: T1DM 15 (n), and T2DM 366 (n). T1DM was excluded from our results. Patients with T2DM were divided into two groups: the control group (not on metformin) consisted of 71 patients, and the group that received metformin (M group) consisted of 295 patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims of the Study<\/jats:title>\n                    <jats:p>To assess serum vitamin B12 levels in patients with T2DM and evaluate the prevalence of vitamin B12 deficiency among those receiving metformin treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The M group significantly decreased vitamin B12 serum levels by 443.56\u2009mg\/mL versus the control group of 541.33\u2009pg\/mL (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.003). The prevalence of patients with vitamin B12 deficiency under the metformin treatment was 23.84%. There was no significant difference in correlation between serum vitamin B12 levels and both doses of metformin and the duration of diabetes mellitus.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Vitamin B12 was significantly decreased in patients with T2DM receiving metformin.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/edm2.70049","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T06:02:21Z","timestamp":1744610541000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of Metformin Therapy on Vitamin\n                    <scp>B12<\/scp>\n                    Levels in Patients With Type 2 Diabetes Mellitus"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4715-1244","authenticated-orcid":false,"given":"Sahar H.","family":"Mahmoud","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Benghazi University  Benghazi Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma R.","family":"Elfargani","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Benghazi University  Benghazi Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagwa","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Benghazi University  Benghazi Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiaza A.","family":"Alhamdi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Benghazi University  Benghazi Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,14]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.33908"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hsr2.2004"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/CLEP.S231184"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21176166"},{"issue":"5","key":"e_1_2_14_6_1","first-page":"579","article-title":"Know the Signs and Symptoms of Diabetes","volume":"140","author":"Ramachandran A.","year":"2014","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.2991\/jegh.k.191028.001"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v12.i9.1401"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.33899\/ajsc.2017.126019"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/6195494"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010009\u20101360\u20105"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc08\u20109025"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010017\u20104318\u2010z"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199508313330902"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqaa257"},{"key":"e_1_2_14_16_1","first-page":"160","article-title":"Vitamin B12 Deficiency in Type 2 Diabetes Patients Using Metformin","volume":"6","author":"Sulaiman G.","year":"2023","journal-title":"AlQalam Journal of Medical and Applied Science"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hnm.2022.200138"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/2042018820980225"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00670.2013"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000017918"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.15537\/smj.2021.42.2.20200662"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.3402\/ljm.v11.31086"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168\u20108227(96)01327\u20105"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15102185"},{"key":"e_1_2_14_25_1","volume-title":"Vitamin B12 Deficiency","author":"Ankar A.","year":"2024"},{"issue":"131","key":"e_1_2_14_26_1","first-page":"107","article-title":"Challenging Clinical Presentations of Pernicious Anemia","volume":"24","author":"Oo T. 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