{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T16:56:00Z","timestamp":1781283360422,"version":"3.54.1"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871222"],"award-info":[{"award-number":["81871222"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570763"],"award-info":[{"award-number":["81570763"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Endocrino Diabet &amp;amp; Metabol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>There is still a lack of enough evidence about Nogo\u2010B levels and vascular complications in patients with type 2 diabetes. Our first aim was to assess the levels of Nogo\u2010B in type 2 diabetes mellitus (T2DM) patients with or without vascular complications (VC). Our second aim was to determine the mechanism by which Nogo\u2010B may protect vasculature using a hyperglycemic HUVEC model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sera or samples of patients with T2DM and subjects without diabetes were collected from the First or Second Affiliated Hospital of Chongqing Medical University. Human umbilical endothelial cells (HUVECs) were purchased and treated with high glucose (HG) and\/or cholesterol (C) before and after Nogo\u2010B knockdown or overexpression. Graphpad and SPSS version 27 software were used for statistical analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      T2DM patients with vascular complications (DM\u2009+\u2009VC) displayed significantly lower levels of Nogo\u2010B when compared with T2DM patients without VC (DM) or subjects without diabetes (NC) (\n                      <jats:italic>p\u2009&lt;<\/jats:italic>\n                      \u20090.001). In addition, lower levels of Nogo\u2010B were independently associated with diabetes and\/or VC in T2DM patients. Nogo\u2010B overexpression reduced the expression of mesenchymal markers (\u03b1\u2010SMA and Collagen\u20101), TGF\u2010\u03b21 and P\u2010smad2\/3, while increasing the expression of endothelial markers (CD31, eNOS and VWF) in HUVECs treated with HG and\/or C.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study has proved that lower levels of Nogo\u2010B are independently associated with VC in T2DM patients. In an in\u00a0vitro model, Nogo\u2010B alleviates endothelial cell injury by affecting TGF\u2010\u03b2 signalling. Further studies are still needed to support or verify our findings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/edm2.70188","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:43:20Z","timestamp":1772991800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Levels and Effects of Nogo\u2010B in Patients With Type 2 Diabetes or Hyperglycemic\n                    <scp>HUVEC<\/scp>\n                    Model"],"prefix":"10.1002","volume":"9","author":[{"given":"Laurent","family":"Irakoze","sequence":"first","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"Roi Khaled Hospital  Bujumbura Burundi"},{"name":"The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linqiang","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanfeng","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8127-3902","authenticated-orcid":false,"given":"Xiangjun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanling","family":"Zeng","sequence":"additional","affiliation":[{"name":"Physical Examination Center, the First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Luo","sequence":"additional","affiliation":[{"name":"Physical Examination Center, the First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulian","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangbin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Banderembako","sequence":"additional","affiliation":[{"name":"Roi Khaled Hospital  Bujumbura Burundi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane","family":"Nkengurutse","sequence":"additional","affiliation":[{"name":"Ministry of Public Health and Fighting AIDS, Epidemiological Emergency Service  Bujumbura Burundi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Lei","sequence":"additional","affiliation":[{"name":"School of Public Health Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4568-8772","authenticated-orcid":false,"given":"Xiaoqiu","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9522-1725","authenticated-orcid":false,"given":"Qingfeng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"e_1_2_15_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1143067"},{"key":"e_1_2_15_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-022-01684-5"},{"key":"e_1_2_15_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2020.107613"},{"key":"e_1_2_15_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306884"},{"key":"e_1_2_15_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/2230-8210.183480"},{"key":"e_1_2_15_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.720466"},{"key":"e_1_2_15_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.04.018"},{"key":"e_1_2_15_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04754-4"},{"key":"e_1_2_15_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/db19-0157"},{"key":"e_1_2_15_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-022-02691-w"},{"key":"e_1_2_15_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.894454"},{"key":"e_1_2_15_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.10.024"},{"key":"e_1_2_15_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000484061"},{"key":"e_1_2_15_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cophys.2023.100678"},{"key":"e_1_2_15_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2016.09.002"},{"key":"e_1_2_15_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-022-01457-0"},{"key":"e_1_2_15_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2017.03.007"},{"key":"e_1_2_15_19_1","doi-asserted-by":"crossref","unstructured":"S.SayedandO.Faruq \u201cCathepsin S Knockdown Suppresses Endothelial Inflammation Angiogenesis and Complement Protein Activity Under Hyperglycemic Conditions In\u00a0Vitro by Inhibiting NF\u2010\u03baB Signaling \u201d24 no.6(2023):5428.","DOI":"10.3390\/ijms24065428"},{"issue":"1","key":"e_1_2_15_20_1","first-page":"621","article-title":"High Glucose Induces Apoptosis of HUVECs in a Mitochondria\u2010Dependent Manner by Suppressing Hexokinase 2 Expression","volume":"18","author":"Zhang J.","year":"2019","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_15_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-024-01264-5"},{"key":"e_1_2_15_22_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc22-S002"},{"issue":"1","key":"e_1_2_15_23_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.2337\/cd23-as01","article-title":"Standards of Care in Diabetes\u20102023 Abridged for Primary Care Providers","volume":"41","author":"American Diabetes Association (ADA) Professional Practice Committee","year":"2022","journal-title":"Clinical Diabetes: A Publication of the American Diabetes Association"},{"key":"e_1_2_15_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpmed.2010.08.019"},{"key":"e_1_2_15_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-018-0787-8"},{"key":"e_1_2_15_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.18"},{"issue":"1","key":"e_1_2_15_27_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.2337\/cd20-as01","article-title":"Standards of Medical Care in Diabetes\u20102020 Abridged for Primary Care Providers","volume":"38","author":"American Diabetes Association (ADA) Professional Practice Committee","year":"2020","journal-title":"Clinical Diabetes: A Publication of the American Diabetes Association"},{"key":"e_1_2_15_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570161117666190502103733"},{"key":"e_1_2_15_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/0141076816688346"},{"key":"e_1_2_15_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehz486"},{"key":"e_1_2_15_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-017-0950-8"},{"key":"e_1_2_15_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-023-01898-1"},{"key":"e_1_2_15_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(24)00110-4"},{"key":"e_1_2_15_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2022.11.002"},{"key":"e_1_2_15_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0320-7_32"},{"key":"e_1_2_15_36_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.62.6.25"},{"key":"e_1_2_15_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2217-9_12"},{"key":"e_1_2_15_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.102633"},{"key":"e_1_2_15_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.paid.2016.06.069"},{"key":"e_1_2_15_40_1","doi-asserted-by":"publisher","DOI":"10.4103\/picr.PICR_87_17"},{"key":"e_1_2_15_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2018.07.008"},{"key":"e_1_2_15_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2023.0490"},{"key":"e_1_2_15_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-024-02527-1"},{"key":"e_1_2_15_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00966.2011"},{"key":"e_1_2_15_45_1","doi-asserted-by":"crossref","unstructured":"X.LinandS. K.Shan \u201cThe Crosstalk Between Endothelial Cells and Vascular Smooth Muscle Cells Aggravates High Phosphorus\u2010Induced Arterial Calcification \u201d13 no.7(2022):650.","DOI":"10.1038\/s41419-022-05064-5"},{"key":"e_1_2_15_46_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101050"},{"key":"e_1_2_15_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-021-01124-0"},{"key":"e_1_2_15_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2016.04.025"},{"key":"e_1_2_15_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907359106"}],"container-title":["Endocrinology, Diabetes &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/edm2.70188","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/edm2.70188","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/edm2.70188","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T15:58:50Z","timestamp":1781279930000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/edm2.70188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/edm2.70188"],"URL":"https:\/\/doi.org\/10.1002\/edm2.70188","archive":["Portico"],"relation":{},"ISSN":["2398-9238","2398-9238"],"issn-type":[{"value":"2398-9238","type":"print"},{"value":"2398-9238","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70188"}}