{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:31:18Z","timestamp":1790105478888,"version":"4.0.1"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":2834,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T00:00:00Z","timestamp":1523923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"RETICS","award":["12\/0042\/0003"],"award-info":[{"award-number":["12\/0042\/0003"]}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012513","name":"CIBERCV","doi-asserted-by":"publisher","award":["CB16\/11\/00261"],"award-info":[{"award-number":["CB16\/11\/00261"]}],"id":[{"id":"10.13039\/501100012513","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Health","doi-asserted-by":"publisher","award":["PI14\/01506"],"award-info":[{"award-number":["PI14\/01506"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Health","doi-asserted-by":"publisher","award":["PI13\/00100"],"award-info":[{"award-number":["PI13\/00100"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["PI14\/01506"],"award-info":[{"award-number":["PI14\/01506"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["PI13\/00100"],"award-info":[{"award-number":["PI13\/00100"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Ischaemic cardiomyopathy (ICM) leads to impaired contraction and ventricular dysfunction, causing high rates of morbidity and mortality. Epigenomics allows the identification of epigenetic signatures in human diseases. We analyse the differential epigenetic patterns of the ASB gene family in ICM patients and relate these alterations to their haemodynamic and functional status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Epigenomic analysis was carried out using 16 left ventricular (LV) tissue samples, eight from ICM patients undergoing heart transplantation and eight from control (CNT) subjects without cardiac disease. We increased the sample size up to 13 ICM and 10 CNT for RNA sequencing and to 14 ICM for pyrosequencing analyses. We found a hypermethylated profile (cg11189868) in the ASB1 gene that showed a differential methylation of 0.26\u0394\u03b2 (P = 0.016). This result was validated by a pyrosequencing technique (0.23\u0394\u03b2, P = 0.048). Notably, the methylation pattern was strongly related to LV ejection fraction (r = \u22120.849, P = 0.008), stroke volume (r = \u22120.929, P = 0.001), and end-systolic and diastolic LV diameters (r = \u22120.743, P = 0.035 for both). ASB1 showed a down-regulation in messenger RNA levels (\u22121.2-fold, P = 0.039).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings link a specific ASB1 methylation pattern to LV structure and performance in end-stage ICM, opening new therapeutic opportunities and providing new insights regarding which is the functionally relevant genome in the ischaemic failing myocardium.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ehf2.12289","type":"journal-article","created":{"date-parts":[[2018,4,18]],"date-time":"2018-04-18T00:11:19Z","timestamp":1524010279000},"page":"732-737","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["<i>ASB1<\/i>\n                    Differential Methylation in Ischaemic Cardiomyopathy: Relationship with Left Ventricular Performance in End-Stage Heart Failure Patients"],"prefix":"10.1093","volume":"5","author":[{"given":"Ana","family":"Ortega","sequence":"first","affiliation":[{"name":"Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe) , Avd. Fernando Abril Martorell, 106, 46026, Valencia ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"Taraz\u00f3n","sequence":"additional","affiliation":[{"name":"Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe) , Avd. Fernando Abril Martorell, 106, 46026, Valencia ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Gil-Cayuela","sequence":"additional","affiliation":[{"name":"Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe) , Avd. Fernando Abril Martorell, 106, 46026, Valencia ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Mart\u00ednez-Dolz","sequence":"additional","affiliation":[{"name":"Heart Failure and Transplantation Unit, Cardiology Department, University and Polytechnic La Fe Hospital , Valencia ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Lago","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Cardiology Research Unit, Department of Cardiology and Institute of Biomedical Research, University Clinical Hospital , Santiago de Compostela ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Ram\u00f3n","family":"Gonz\u00e1lez-Juanatey","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Cardiology Research Unit, Department of Cardiology and Institute of Biomedical Research, University Clinical Hospital , Santiago de Compostela ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Epigenomic Unit, Health Research Institute La Fe , Valencia ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Portol\u00e9s","sequence":"additional","affiliation":[{"name":"Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe) , Avd. Fernando Abril Martorell, 106, 46026, Valencia ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Rosell\u00f3-Llet\u00ed","sequence":"additional","affiliation":[{"name":"Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe) , Avd. Fernando Abril Martorell, 106, 46026, Valencia ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Rivera","sequence":"additional","affiliation":[{"name":"Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe) , Avd. 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