{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T19:10:49Z","timestamp":1769281849726,"version":"3.49.0"},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":1953,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Grupo Quiron Salud Hospitals, Spain"},{"name":"Grupo Quiron Salud Hospitals, Spain"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Cell therapy regenerative potential is hindered by cell access to the infarct zone. We studied function recovery at the scar zone and its impact in global left ventricular function after intracoronary injection of haematopoietic precursor cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Haematopoietic precursor cells were obtained by blood apheresis in patients with an old myocardial infarction, and the presence of CD34+ and CD133+ cells was quantified. Left ventricular function, volumes, and infarct zone segmental motion were measured by magnetic resonance imaging (MRI) and echo left ventricular segmental strain (LVSS). The aphaeresis product was administered to 20 patients in the coronary artery responsible for the myocardial infarction. High cell yield in blood aphaeresis product allowed us to inject a high number of cells in most patients. Three patients were excluded because of insufficient CD133+ cell number, and one more patient was excluded because of artefacts in MRI images. The remaining 16 patients were compared with 16 controls. After 1 year, infarct zone reduction was related to the number of CD133+ (R = 0.53; P %3C 0.05) and CD34+ (R = 0.63; P %3C 0.01) cells injected. The number of CD133+ cells injected was also related to an improvement in LVSS (R = 0.62; P %3C 0.01). In turn, scar zone reduction was related to an improvement in LVSS (R = 0.64; P %3C 0.01). End-diastolic volume showed a reduction at follow-up in the treated group when compared with control patients. MRI infarct area segments systolic thickness increase improved after treatment in treated patients [expressed as median (interquartile range)] [0.42 (\u22120.38 to 1.14) vs. 1.06 (\u22120.10 to 2.12) mm; P %3C 0.01], but not in controls [2.02 (0.75 to 3.4) vs. 1.91 (0.77\u20133.17) mm; P = not significant (n.s.)]. In cell therapy patients, the borders of the infarct zone, but not the core, showed a significant recovery [proximal rim: 0.48 (\u22120.18 to 1.33) vs. 1.07 (0.22\u20132.40) mm; P %3C 0.05, distal rim: 0.75 (0.26\u20131.40) vs. 1.76 (0.65\u20132.86) mm; P %3C 0.05, and core: 0.36 (\u22120.33 to 1.20) vs. 0.60 (\u22120.18 to 1.62) mm; P = n.s.]. That improvement was not observed in the control group [proximal rim: 1.20 (0.33\u20132.53) vs. 0.82 (\u22120.13 to 1.65) mm; P = n.s., distal rim: 1.24 (0.80\u20131.72) vs. 0.96 (0.19\u20131.81) mm; P = n.s., and core: 0.30 (\u22120.42 to 1.64) vs. 0.07 (\u22120.60 to 1.20) mm; P = n.s.]. Only small size infarcts showed a complete recovery in the cell therapy patients [systolic thickness increase post-treatment increment in infarcts \u22646 segments vs. &amp;gt;6 segments affected: 0.28 (\u22120.19 to 0.71) vs. \u22121.21 (\u22122.60 to \u22120.53) mm; P %3C 0.01].<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Intracoronary injection of peripheral blood-derived haematopoietic precursor cells produces a complete recovery of the borders and partial regeneration of the infarct core, which is directly related to the number of CD133+ and CD34+ cells injected. Cell therapy infarct zone regeneration prevents ventricular remodelling by preserving segmental contractility and halting left ventricular dilatation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ehf2.12911","type":"journal-article","created":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T00:45:05Z","timestamp":1597452305000},"page":"2962-2971","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Intracoronary Injection of Haematopoietic Precursor Cells Regenerates the Borders, but Not the Core, of Old Myocardial Scars"],"prefix":"10.1093","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9825-0320","authenticated-orcid":false,"given":"Alvaro","family":"Merino","sequence":"first","affiliation":[{"name":"Clinica Quir\u00f3n Rotger , Palma ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoni","family":"Gaya","sequence":"additional","affiliation":[{"name":"Grupo Terapia Celular e Ingenieria Tisular (TERCIT), Instituto de Investigaciones Sanitarias Illes Balears (IDISBA) , Palma ,","place":["Spain"]},{"name":"Fundaci\u00f3 Banc de Sang i Teixits de les Illes Balears , Palma ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Calvo","sequence":"additional","affiliation":[{"name":"Grupo Terapia Celular e Ingenieria Tisular (TERCIT), Instituto de Investigaciones Sanitarias Illes Balears (IDISBA) , Palma ,","place":["Spain"]},{"name":"Fundaci\u00f3 Banc de Sang i Teixits de les Illes Balears , Palma ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramon","family":"Rotger","sequence":"additional","affiliation":[{"name":"Clinica Quir\u00f3n Rotger , Palma ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana","family":"Nu\u00f1ez","sequence":"additional","affiliation":[{"name":"Clinica Quir\u00f3n Rotger , Palma ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,8,14]]},"reference":[{"key":"2026012114062995600_ehf212911-bib-0001","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/j.jtcvs.2008.03.068","article-title":"Long-term cardiac remodeling after salvage partial left ventriculectomy in an infant with anomalous left coronary artery from the pulmonary artery","volume":"137","author":"Westaby","year":"2009","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2026012114062995600_ehf212911-bib-0002","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/35070587","article-title":"Bone marrow cells regenerate infarcted myocardium","volume":"410","author":"Orlic","year":"2001","journal-title":"Nature"},{"key":"2026012114062995600_ehf212911-bib-0003","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1093\/eurheartj\/ehw113","article-title":"Position paper of the European Society of Cardiology Working Group Cellular Biology of the Heart: cell-based therapies for myocardial repair and regeneration in ischemic heart disease and heart failure","volume":"37","author":"Madonna","year":"2016","journal-title":"Eur Heart J"},{"key":"2026012114062995600_ehf212911-bib-0004","doi-asserted-by":"crossref","first-page":"3061","DOI":"10.1093\/eurheartj\/ehv390","article-title":"Randomized trial of combination cytokine and adult autologous bone marrow progenitor cell administration in patients with non-ischemic dilated cardiomyopathy: the REGENERATE- DCM clinical trial","volume":"36","author":"Hamshere","year":"2015","journal-title":"Eur Heart J"},{"key":"2026012114062995600_ehf212911-bib-0005","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1161\/CIRCRESAHA.117.311302","article-title":"Rendon meta-analysis of cell therapy studies in heart failure and acute myocardial infarction","volume":"123","author":"Gy\u00f6ngy\u00f6si","year":"2018","journal-title":"Circ Res"},{"key":"2026012114062995600_ehf212911-bib-0006","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ahj.2010.09.025","article-title":"CD34+ cell infusion after ST elevation myocardial infarction is associated with improved perfusion and is dose dependent","volume":"161","author":"Quyyumi","year":"2011","journal-title":"Am Heart J"},{"key":"2026012114062995600_ehf212911-bib-0007","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1002\/(SICI)1097-0320(19980415)34:2<61::AID-CYTO1>3.0.CO;2-F","article-title":"Single platform flow cytometric absolute CD34\u00fe cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering","volume":"34","author":"Keeney","year":"1998","journal-title":"Cytometry"},{"key":"2026012114062995600_ehf212911-bib-0008","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1016\/j.echo.2005.10.005","article-title":"Recommendations for chamber quantification: a report from the American Society of Echocardiography's Guidelines and Standards Committee and the Chamber Quantification Writing Group, developed in conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology","volume":"12","author":"Lang","year":"2005","journal-title":"J Am Soc Echocardiogr"},{"key":"2026012114062995600_ehf212911-bib-0009","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.jcyt.2013.08.005","article-title":"Mobilization and engraftment of peripheral blood stem cells in healthy related donors %3E55years old","volume":"16","author":"Motllo","year":"2014","journal-title":"Cytotherapy"},{"key":"2026012114062995600_ehf212911-bib-0010","doi-asserted-by":"crossref","first-page":"2990","DOI":"10.1016\/j.jacc.2019.03.503","article-title":"Pro-angiogenic macrophage phenotype to promote myocardial repair","volume":"73","author":"Ferraro","year":"2019","journal-title":"J Am Coll Cardiol"},{"key":"2026012114062995600_ehf212911-bib-0011","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1161\/CIRCULATIONAHA.105.541268","article-title":"Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction","volume":"113","author":"Iwasaki","year":"2006","journal-title":"Circulation"},{"key":"2026012114062995600_ehf212911-bib-0012","doi-asserted-by":"crossref","first-page":"2532","DOI":"10.1093\/eurheartj\/ehx248","article-title":"Global position paper on cardiovascular regenerative medicine","volume":"38","author":"Fernandez-Aviles","year":"2017","journal-title":"Eur Heart J"},{"key":"2026012114062995600_ehf212911-bib-0013","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1161\/CIRCULATIONAHA.105.593038","article-title":"Proarrhythmic potential of mesenchymal stem cell transplantation revealed in an in vitro coculture model","volume":"113","author":"Chang","year":"2006","journal-title":"Circulation"}],"container-title":["ESC Heart Failure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fehf2.12911","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ehf2.12911","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ehf2.12911","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/eschf\/article-pdf\/7\/5\/2962\/65807201\/eschf_7_5_2962.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/eschf\/article-pdf\/7\/5\/2962\/65807201\/eschf_7_5_2962.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T19:06:59Z","timestamp":1769022419000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/eschf\/article\/7\/5\/2962\/8372114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,14]]},"references-count":13,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,8,14]]},"published-print":{"date-parts":[[2020,10,1]]}},"URL":"https:\/\/doi.org\/10.1002\/ehf2.12911","relation":{},"ISSN":["2055-5822"],"issn-type":[{"value":"2055-5822","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,10]]},"published":{"date-parts":[[2020,8,14]]},"assertion":[{"value":"2020-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}