{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:10:08Z","timestamp":1791641408714,"version":"4.3.4"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2015,2,10]],"date-time":"2015-02-10T00:00:00Z","timestamp":1423526400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100000850","name":"National Heart and Lung Institute","doi-asserted-by":"publisher","award":["HHSN268200417175C"],"award-info":[{"award-number":["HHSN268200417175C"]}],"id":[{"id":"10.13039\/501100000850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The significance of detection of Trypanosoma cruziDNA in blood of antibody-positive patients for risk of development of Chagas heart disease is not well established. The objective of this study was to compare detection of T. cruziDNA with known clinical and laboratory markers of Chagas cardiomyopathy (CC) severity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This is a case\u2013control study nested within a retrospective cohort developed in Brazil to understand the natural history of Chagas disease. The study enrolled 499 T. cruzi seropositive blood donors (SP-BD) and 488 frequency matched seronegative control donors (SN-BD) who had donated between 1996 and 2002, and 101 patients with clinically diagnosed CC. In 2008\u20132010 all enrolled subjects underwent a health questionnaire, medical examination, electrocardiograms and echocardiograms and polymerase chain reaction (PCR) analyses. A blinded panel of three cardiologists adjudicated the outcome of CC. Trypanosoma cruzi kinetoplast minicircle sequences were amplified by real-time PCR using an assay with a sensitivity of one parasite per 20\u2009mL of blood. All testing was performed on coded samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Rates of PCR detection of T. cruziDNA were significantly (P\u2009=\u20090.003) higher in CC patients and SP-BD diagnosed with CC (79\/105 [75.2 %]) compared with SP-BD without CC (143\/279 [51.3%]). The presence of parasitaemia was significantly associated with known markers of disease progression such as QRS and QT interval duration, lower left ventricular ejection fraction, higher left ventricular index mass, and elevated troponin and NTpro-BNP levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Trypanosoma cruziPCR positivity is associated with presence and severity of cardiomyopathy, suggesting a direct role of parasite persistence in disease pathogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ejhf.220","type":"journal-article","created":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T13:57:30Z","timestamp":1423749450000},"page":"416-423","source":"Crossref","is-referenced-by-count":59,"title":["Detection of\n                    <i>Trypanosoma Cruzi<\/i>\n                    DNA in Blood by PCR is Associated with Chagas Cardiomyopathy and Disease Severity"],"prefix":"10.1093","volume":"17","author":[{"given":"E. C.","family":"Sabino","sequence":"first","affiliation":[{"name":"Department of Infectious Disease and Institute of Tropical Medicine, University of S\u00e3o Paulo , S\u00e3o Paulo ,","place":["Brazil"]},{"name":"Funda\u00e7\u00e3o Pr\u00f3-Sangue Hemocentro de Sao Paulo , S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas and Faculdade de Medicina, Universidade Federal de Minas Gerais , Minas Gerais ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. H","family":"Lee","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute , 270 Masonic Avenue, San Francisco, CA, 94118 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. L.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Campus Dona Lindu da Universidade Federal de S\u00e3o Jo\u00e3o del-Rei , Minas Gerais ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. B.","family":"Carneiro-Proietti","sequence":"additional","affiliation":[{"name":"Hemominas and Faculdade da Sa\u00fade e Ecologia Humana\u2013FASEH , Minas Gerais ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P.","family":"Antunes","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade de Montes Claros , Minas Gerais ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. M.","family":"Menezes","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade de Montes Claros , Minas Gerais ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. M.","family":"Ianni","sequence":"additional","affiliation":[{"name":"Cardiomyopathy Unit of the Heart Institute (InCor) da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. M.","family":"Salemi","sequence":"additional","affiliation":[{"name":"Cardiomyopathy Unit of the Heart Institute (InCor) da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Nastari","sequence":"additional","affiliation":[{"name":"Cardiomyopathy Unit of the Heart Institute (InCor) da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Cardiomyopathy Unit of the Heart Institute (InCor) da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Sachdev","sequence":"additional","affiliation":[{"name":"National Heart, Lung and Blood Institute , Bethesda, MD ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. M.","family":"Carrick","sequence":"additional","affiliation":[{"name":"Westat, Inc. , Rockville, MD ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Deng","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute , 270 Masonic Avenue, San Francisco, CA, 94118 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Wright","sequence":"additional","affiliation":[{"name":"Westat, Inc. , Rockville, MD ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. T.","family":"Gon\u00e7alez","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute , 270 Masonic Avenue, San Francisco, CA, 94118 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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