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The degree of cross\u2010reactivity was reduced after passage of the antisera through Sepharose columns containing covalently bound heterologous antigen.<\/jats:p><jats:p>The cross\u2010reactivity between pepsin and pepsinogen was quantitated by several methods: (a) by precipitin reaction, (b) by measuring the amount of antibodies obtained after passing sera on Sepharose columns to which the heterologous antigen was covalently bound, (c) by measuring the inhibition of the binding of <jats:sup>125<\/jats:sup>I\u2010labeled antigen to antisera by unlabeled antigens and (d) by measuring the amount of heterologous <jats:sup>125<\/jats:sup>I\u2010labeled antigen which could be added to an antiserum already saturated with the homologous <jats:sup>125<\/jats:sup>I\u2010labeled antigen.<\/jats:p><jats:p>The results indicated that anti\u2010pepsinogen antisera contained a class of antibodies which could only bind pepsin and not the homologous antigen, pepsinogen. These results suggest that in rabbits immunized with pepsinogen, antibodies were produced against determinants which are not present in native pepsinogen.<\/jats:p>","DOI":"10.1002\/eji.1830010603","type":"journal-article","created":{"date-parts":[[2007,2,27]],"date-time":"2007-02-27T21:37:00Z","timestamp":1172612220000},"page":"421-426","source":"Crossref","is-referenced-by-count":1,"title":["Cross\u2010reacting anti\u2010pepsin and anti\u2010pepsinogen antisera"],"prefix":"10.1002","volume":"1","author":[{"given":"M.","family":"Bustin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Conway\u2010Jacobs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-65123-6_3"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)81244-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00902a006"},{"key":"e_1_2_1_5_2","first-page":"634","volume":"3","author":"Schlamovitz M.","year":"1964","journal-title":"Biochemistry"},{"key":"e_1_2_1_6_2","first-page":"35","volume":"21","author":"Freedberg I. 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