{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T01:09:35Z","timestamp":1774660175680,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":11611,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1974,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spleen cells from mice immunized intravenously with attenuated ectromelia virus were cytotoxic for target cells infected with virulent ectromelia virus in the absence of exogenous complement; cytotoxicity was measured by <jats:sup>51<\/jats:sup>Cr release from target cells. L929 cells were the most sensitive target cells, but the effect could also be demonstrated with P\u2010815 mastocytoma cells and chick embryo cells; mouse embryo cells were unsatisfactory. Hyperimmune anti\u2010ectromelia serum and complement was not significantly cytotoxic although fluorescein\u2010conjugated antiserum stained virus\u2010infected L929 cells.<\/jats:p><jats:p>Release of <jats:sup>51<\/jats:sup>Cr label from ectromelia\u2010immune spleen cells (at a spleen cell: target cell ratio of 100: 1) increased in a linear manner with time, until a plateau was reached at 20\u201324 hours. Cytotoxicity appeared to be specific in that ectromelia\u2010immune spleen cells killed ectromelia\u2010infected L929 cells, but not uninfected L929 cells, whereas spleen cells from mice immunized with <jats:italic>Listeria monocytogenes<\/jats:italic> (a potent stimulus for cell\u2010mediated immunity) or from normal mice, did not kill ectromelia\u2010infected L929 cells. Spleen cells from mice immunized with vaccinia virus (a poxvirus closely related to ectromelia) were cytotoxic for ectromelia\u2010infected L929 cells.<\/jats:p><jats:p>Spleen cells from mice immunized with ectromelia virus had acquired cytotoxic activity by 2 days after immunization. Cytotoxic potency reached a peak at 6 days and had declined to a low level by day 10. The potential significance of the phenomenon in relation to control of viral infection is discussed.<\/jats:p>","DOI":"10.1002\/eji.1830040202","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T00:07:01Z","timestamp":1172621221000},"page":"63-67","source":"Crossref","is-referenced-by-count":81,"title":["Cell\u2010mediated cytotoxicity against ectromelia virus\u2010infected target cells. I. Specificity and kinetics"],"prefix":"10.1002","volume":"4","author":[{"given":"I.","family":"Gardner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narelle A.","family":"Bowern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. V.","family":"Blanden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1949.1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"341","DOI":"10.4049\/jimmunol.63.4.341","volume":"63","author":"Fenner F.","year":"1949","journal-title":"J. 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