{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T00:05:31Z","timestamp":1698969931216},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":11277,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1975,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies using synthetic immunogenic molecules containing two haptenic peptides (the NH<jats:sub>2<\/jats:sub>\u2010terminal heptapeptide and the COOH\u2010terminal pentapeptide of oxidized ferredoxin (O\u2010Fd) from <jats:italic>C. pasteurinaum<\/jats:italic> ) have shown that both peptides individually are capable of initiating lymphocyte transformation and inhibiting migration in populations of lymphocytes from O\u2010Fd\u2010sensitized guinea pigs. While migration inhibition could readily be stimualted by single haptenic peptides, lymphocytes transforamtion appeared to be more easily induced by molecules containing two or more haptenic peptides (these could be identical or different) (Kelly, B., Levy, J. G. and Hull, D., <jats:italic>Eur. J. Immunol.<\/jats:italic>, 1973. <jats:italic>3<\/jats:italic>: 574). If lymphocyte transformation is a T cell\u2010mediated phenomenon, these observations indicate the possibility of T cell\u2010T cell interaction. The two haptenic peptides (designated \u201eN\u201d\ufe01 and \u201eC\u201d\ufe01) were synthesized and conjugated to succinylated bovine serum albumin (S\u2010BSA), forming the cojugates N\u2010S\u2010BSA and C\u2010S\u2010BSA, repectively. These conjugates were labled to high specific activity with <jats:sup>125<\/jats:sup>iodine and were used in and \u201eantigen suicide\u201d\ufe01 procedure to treat guinea pig lymph node cell preparation previously sensitized to 0\u2010Fd adn keyhole limpet hemocyanin (KLH). Cell population exposed to either <jats:sup>125<\/jats:sup>I\u2010labeled N\u2010S\u2010BSA or <jats:sup>125<\/jats:sup>I\u2010labeled C\u2010S\u2010BCA demonstrated decreased lymphocytes transformation in the presence of O\u2010Fd but not in hte presence of KLH. These results indicate specific cell inactivation cell transformation. Experiments performed by mixing <jats:sup>125<\/jats:sup>I\u2010labeled N\u2010S\u2010BSA\u2010treated cells with <jats:sup>125<\/jats:sup>I\u2010labeled C\u2010S\u2010BSA\u2010treated cells were successful in partially restoring the response to O\u2010Fd and suggest possible synergy between N and C determinant binding cells in the cellular immune response to O\u2010Fd. Evidence from B cell depletion studies suggests that this is a T cell\u2010T cell interaction.<\/jats:p>","DOI":"10.1002\/eji.1830050114","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T00:39:43Z","timestamp":1172623183000},"page":"65-69","source":"Crossref","is-referenced-by-count":4,"title":["The effect of specific cell inactivation on the cellular immune response to ferreodoxin peptides"],"prefix":"10.1002","volume":"5","author":[{"given":"T.","family":"Pearson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Levy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Kilburn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830010202"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830010204"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/2261257a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(72)90164-5"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(72)90086-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830030910"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/2221291a0"},{"key":"e_1_2_1_9_2","volume-title":"Developmental Aspects of Antibody Formation and Structure","author":"Mitchison N. 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