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The reaction occurred in the absence of detectable amounts of antibodies, since an agammaglobulinemic patient's serum also supported complement activation, and the results were unaltered by absorption of normal human serum. Trypsin treatment of lymphocytes to remove possible complement activators bound to the cell membrane, did not have any effect on the complement activation by lymphocytes. Complement membrane fluorescence was abolished in the presence of EDTA or ethyleneglycolbis(aminoethylether) tetraacetate (EGTA) supplemented by Mg<jats:sup>++<\/jats:sup>ions indicating that the classical pathway of complement activation was involved in the reaction. Experiments performed with T and B\u2010purifiedpopulations showed that the phenomenon was confined to the B cell fraction. Complement deposition did not diminish the number of EAC rosettes, and viability remained unchanged after exposure of lymphocytes to normal human serum.<\/jats:p>","DOI":"10.1002\/eji.1830090115","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T10:11:04Z","timestamp":1172657464000},"page":"72-76","source":"Crossref","is-referenced-by-count":5,"title":["Antibody\u2010independent activation of complement by human peripheral B lymphocytes"],"prefix":"10.1002","volume":"9","author":[{"given":"Carmen","family":"Guti\u00e9rrez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus","family":"Vega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Kreisler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"348","DOI":"10.4049\/jimmunol.113.1.348","volume":"113","author":"Platts\u2010Mills T.","year":"1974","journal-title":"J. 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