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Lymphocytes, primed <jats:italic>in vitro<\/jats:italic> to and restimulated with cells of a DLA\u2010identical sib, did not react to these specific cells, in contrast to normal proliferation to other stimulators. However, lymphocytes obtained after <jats:italic>in vivo<\/jats:italic> sensitization against a DLA\u2010identical donor showed increased reactivity to the donor in approximately half of the recipients. In comparison to primary MLC responses between DLA\u2010mismatched individuals, the [<jats:sup>3<\/jats:sup>H] thymidine uptake was less important in this reaction, and peak incorporation was usually not accelerated. This response appeared to be specific in that responsiveness to third\u2010party cells was not increased, and, alternatively, immunization against a DLA haplo\u2010identical donor did not modify reactivity to DLA\u2010identical cells. This increased reactivity after alloimmunization may reflect differences in minor histocompatibility antigens although the skin graft survival time was not significantly shorter in MLC responders than in nonresponder recipients. It seems that <jats:italic>in vivo<\/jats:italic> priming is required to expand the number of cells specific for these antigens and to enable detection of proliferation in MLC.<\/jats:p>","DOI":"10.1002\/eji.1830100907","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T13:03:23Z","timestamp":1172667803000},"page":"693-697","source":"Crossref","is-referenced-by-count":2,"title":["Change in mixed lymphocyte culture reactivity following allosensitization between DLA\u2010identical dog sibs"],"prefix":"10.1002","volume":"10","author":[{"given":"Jean\u2010Claude","family":"Gluckman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60320-X"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1978.121"},{"key":"e_1_2_1_4_2","first-page":"141","volume":"10","author":"Gluckman E.","year":"1978","journal-title":"Transplant. 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