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T<jats:sub>s<\/jats:sub>S and T<jats:sub>s<\/jats:sub>N can suppress cytotoxic T lymphocyte (CTL) induction upon transfer to fresh (second) MLC stimulated by the same alloantigens as in the first MLC (T<jats:sub>s<\/jats:sub>S or T<jats:sub>s<\/jats:sub>N) or by third\u2010party alloantigens (T<jats:sub>s<\/jats:sub>N only). Evidence that T<jats:sub>s<\/jats:sub>S and T<jats:sub>s<\/jats:sub>N functions are performed by different T cell sets has been restricted to radioresistance of the former but not the latter. Separation of T<jats:sub>s<\/jats:sub>S from CTL has proven even more difficult. Methods are reported here which have allowed <jats:italic>in vitro<\/jats:italic> induction and functional separation of T<jats:sub>s<\/jats:sub>S from CTL and T<jats:sub>s<\/jats:sub>N in a totally allogeneic system. T<jats:sub>s<\/jats:sub>S are resistant to combined exposure to pyrilamine, a histamine<jats:sub>1<\/jats:sub> antagonist and local anesthetic, during suppressor cell induction, and to X or \u03b3 irradiation thereafter, while CTL precursors (CTL\u2010P) and T<jats:sub>s<\/jats:sub>N are more sensitive to such treatments. This allowed us to use these treatments to generate T<jats:sub>s<\/jats:sub>S that are not contaminated with functional CTL, CTL\u2010P or T<jats:sub>s<\/jats:sub>N. These data show that T<jats:sub>s<\/jats:sub>S regulate CTL induction by interacting with responding cell populations, rather than by cytotoxic depletion of stimulator cells.<\/jats:p>","DOI":"10.1002\/eji.1830120505","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T18:35:09Z","timestamp":1172687709000},"page":"380-386","source":"Crossref","is-referenced-by-count":16,"title":["Regulation of <i>in vitro<\/i> cytotoxic T lymphocyte generation. II. 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