{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T00:25:53Z","timestamp":1698971153568},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":8746,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>(NZB \u00d7 SJL)F<jats:sub>1<\/jats:sub> (NS) female mice develop a marked hypertrophy of the thymus in the course of aging. The age\u2010dependent evolution of the intrathymic lymphocyte population of these mice was monitored in comparison with that of the immunologically normal BALB\/c and C57BL\/6 strains. The expression of several lymphocyte markers on the surface of the thymic cells was quantitated using single\u2010 and two\u2010color flow cytofluorometry analysis. Two main cell types could thus be identified in the thymus of 3\u2010month\u2010old mice: a major subset of bright Thy\u20101<jats:sup>+<\/jats:sup>, Lyt\u20101<jats:sup>+<\/jats:sup>2<jats:sup>\u2212<\/jats:sup>, bright peanut agglutinin (PNA)<jats:sup>+<\/jats:sup> lymphocytes and a minor subset of dull Thy\u20101<jats:sup>+<\/jats:sup>, Lyt\u20101<jats:sup>+<\/jats:sup>2<jats:sup>\u2212<\/jats:sup>, dull PNA<jats:sup>+<\/jats:sup> lymphocytes. In BALB\/c and C57BL\/6 mice, the frequencies of these cell types did not significantly vary between 3 and 18 months of age, despite a drop of thymic cellularity. In contrast, in NS female mice the proportion as well as the absolute number of dull Thy\u20101<jats:sup>+<\/jats:sup>, Lyt\u20101<jats:sup>+<\/jats:sup>2<jats:sup>\u2212<\/jats:sup>, dull PNA<jats:sup>+<\/jats:sup> cells increased while those of bright Thy\u20101<jats:sup>+<\/jats:sup>, Lyt<jats:sup>\u2212<\/jats:sup>1<jats:sup>+<\/jats:sup>2<jats:sup>\u2212<\/jats:sup>, bright PNA<jats:sup>+<\/jats:sup> cells diminished during the same period. The emergence of new cell types could also be noted in the thymus of aging NS female mice. Thus, in addition to the appearance of dull PNA<jats:sup>+<\/jats:sup>, Lyt\u20102<jats:sup>+<\/jats:sup> cells and of bright PNA<jats:sup>+<\/jats:sup> cells devoid of T cell markers, there was a high frequency of non\u2010T cells bearing surface immunogloublin, Ia antigen and receptors for Fc. These data indicate that the thymic hypertrophy of NS female mice reflects the intrathymic accumulation of large numbers of phenotypically mature T and B cells. Such alterations were not detectable in NS male mice.<\/jats:p>","DOI":"10.1002\/eji.1830120805","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T19:00:55Z","timestamp":1172689255000},"page":"648-652","source":"Crossref","is-referenced-by-count":4,"title":["Age\u2010related alterations of the lymphocyte population in the thymus of (NZB \u00d7 SJL)F<sub>1<\/sub> mice: Analysis by flow cytofluorometry"],"prefix":"10.1002","volume":"12","author":[{"given":"Francis","family":"Dumont","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"Habbersett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-46044-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-2541-3_5"},{"key":"e_1_2_1_4_2","first-page":"357","volume-title":"Electrophoresis 78","author":"Dumont F.","year":"1978"},{"key":"e_1_2_1_5_2","first-page":"63","volume":"41","author":"Dumont F.","year":"1980","journal-title":"Clin. 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