{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T22:20:01Z","timestamp":1759702801200},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":8355,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1983,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When normal human B cells from blood, tonsil or spleen are cultured with 20\u201330% autologous or allogeneic T cells and mitogen, the majority of cells rosette with sheep erythrocytes (E) after 3\u20135 days in culture. These E<jats:sup>+<\/jats:sup> cells are of transformed appearance and several points indicate that many are derived from B cells. Some simultaneously possess surface immunoglobulin (sIg) or a B cell antigen [detected with BA1 monoclonal antibody (mAb)] and E receptor. Many do not stain with anti\u2010T cell mAb (UCHT1, OKT4, OKT8), while cultured T cells continue to express these antigens. Furthermore, many E<jats:sup>+<\/jats:sup> cells appear when the original T cells have been irradiated. All the cultured E<jats:sup>+<\/jats:sup> cells stain with OKT11, an mAb against the E receptor, and their positivity cannot therefore be attributed to mitogen\u2010induced nonspecific stickiness. The E positivity of the B cells was shown to be endogenous since passive acquisition of E receptor shed by T cells was excluded in a number of ways; no phenotypic changes were observed when supernatants from cultures containing many E<jats:sup>+<\/jats:sup> sIg<jats:sup>+<\/jats:sup> non\u2010T cells were added to B cells, or when the B and T cells were separated by a Millipore membrane; and E receptor was re\u2010expressed after stripping with trypsin or pronase. The intimate presence of T cells is essential for the expression of E receptors by B cells, and this helper capacity was shown to reside within the OKT4\u2010defined helper T cell subpopulation. The significance of the expression of E receptor by B cells is discussed in relation to the recent <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> demonstration of E<jats:sup>+<\/jats:sup> sIg<jats:sup>+<\/jats:sup> cells in certain leukemias and in relation to the specificity of E rosetting as a marker of T cells.<\/jats:p>","DOI":"10.1002\/eji.1830130506","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T20:23:09Z","timestamp":1172694189000},"page":"379-382","source":"Crossref","is-referenced-by-count":14,"title":["Normal human B cells express endogenous sheep erythrocyte (E) receptor after appropriate <i>in vitro<\/i> culture"],"prefix":"10.1002","volume":"13","author":[{"given":"Kingston H. G.","family":"Mills","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin P.","family":"Worman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Cawley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830110412"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1182\/blood.V60.2.373.373","volume":"60","author":"Schroff R. W.","year":"1982","journal-title":"Blood"},{"key":"e_1_2_1_4_2","unstructured":"Armitage R. J. Worman C. P. Mills K. H. G.andCawley J. C. Scand. J. Hematol.1982 in press."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19810401)47:7<1800::AID-CNCR2820470713>3.0.CO;2-P"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/286166a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1182\/blood.V59.5.895.895","volume":"59","author":"Worman C. P.","year":"1982","journal-title":"Blood"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1982.tb03882.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1182\/blood.V52.6.1132.1132","volume":"52","author":"Burns G. F.","year":"1978","journal-title":"Blood"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830120115"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(81)90019-0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4049\/jimmunol.126.1.83","volume":"126","author":"Abramson C. S.","year":"1981","journal-title":"J. Immunol."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(77)90065-5"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(78)90123-0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1182\/blood.V55.5.784.784","volume":"55","author":"Cawley J. C.","year":"1980","journal-title":"Blood"},{"key":"e_1_2_1_16_2","first-page":"76","volume":"39","author":"Burns G. F.","year":"1980","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/259124a0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(73)90007-X"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830130506","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830130506","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T21:46:24Z","timestamp":1695764784000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830130506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,1]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1983,1]]}},"alternative-id":["10.1002\/eji.1830130506"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830130506","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,1]]}}}