{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T05:40:16Z","timestamp":1697866816752},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":7990,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1984,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Single\u2010 and dual\u2010parameter fluorescence\u2010activated cell sorter analyses of complement receptor\u2010positive and \u2010negative (CR<jats:sup>+<\/jats:sup> and CR<jats:sup>\u2212<\/jats:sup>) splenic B cells of normal and <jats:italic>xid<\/jats:italic> immunodeficient mice were performed to determine the frequencies of surface (s) IgM<jats:sup>+<\/jats:sup> and sIgD<jats:sup>+<\/jats:sup> cells, as well as the quantities of sIgM and sIgD on these cells. Single parameter analysis revealed identical frequencies of sIgM<jats:sup>+<\/jats:sup> and sIgD<jats:sup>+<\/jats:sup> cells in the CR<jats:sup>+<\/jats:sup> B cell population of normal mice. In contrast, the percentage of sIgM<jats:sup>+<\/jats:sup> cells exceeded that of sIgD<jats:sup>+<\/jats:sup> cells in the CR<jats:sup>\u2212<\/jats:sup> normal and CR<jats:sup>+<\/jats:sup> <jats:italic>xid<\/jats:italic> B cell population to a similar extent. CR<jats:sup>\u2212<\/jats:sup> <jats:italic>xid<\/jats:italic> B cells showed an even greater discrepancy between sIgM<jats:sup>+<\/jats:sup> and sIgD<jats:sup>+<\/jats:sup> cells.<\/jats:p><jats:p>Dual\u2010parameter analysis of CR<jats:sup>+<\/jats:sup> normal B cells showed the typical sIg phenotype of mature B cells, <jats:italic>i.e.<\/jats:italic> an intermediate density of sIgM and a high density of sIgD. The CR<jats:sup>+<\/jats:sup> <jats:italic>xid<\/jats:italic> B cell population displayed an increase in cells with high sIgM and no\u2010to\u2010low sIgD and a decrease in cells with intermediate sIgM and high sIgD when compared to CR<jats:sup>+<\/jats:sup> normal cells. CR<jats:sup>\u2212<\/jats:sup> B cells from normal and <jats:italic>xid<\/jats:italic> immunodeficient mice were similar in that both consisted largely of a cell population with low sIgM and no\u2010to\u2010low sIgD. However, the CR<jats:sup>\u2212<\/jats:sup> normal B cell population also contained cells with intermediate sIgM and high sIgD, which were lacking in the CR<jats:sup>\u2212<\/jats:sup> <jats:italic>xid<\/jats:italic> cell population. Similar results were obtained when CR<jats:sup>+<\/jats:sup> and CR<jats:sup>\u2212<\/jats:sup> B cells from normal and <jats:italic>xid<\/jats:italic> mice were purified from a small resting B cell population, isolated by elutriation. This indicates that the sIg phenotype of CR<jats:sup>\u2212<\/jats:sup> cells is not due to the presence of large cells which have lost sIgD during activation.<\/jats:p>","DOI":"10.1002\/eji.1830140410","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T21:05:02Z","timestamp":1172696702000},"page":"329-335","source":"Crossref","is-referenced-by-count":1,"title":["Surface immunoglobulin phenotype of complement receptor\u2010positive and complement receptor\u2010negative B cells of normal and <i>xid<\/i> immunodeficient mice"],"prefix":"10.1002","volume":"14","author":[{"given":"Tullia","family":"Lindsten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred D.","family":"Finkelman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig B.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birger","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irwin","family":"Scher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"2","volume":"33","author":"Scher I.","year":"1982","journal-title":"Adv. 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