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Subsequently, a quantitative measure of pre\u2010B cell proliferation was provided by examining marrow cells by immunofluorescence labeling for the absolute number of pre\u2010B cells, identified by the presence of cytoplasmic \u03bc chains (c\u03bc) without surface \u03bc (s\u03bc), which had been arrested in meta\u2010phase. In anti\u2010IgM\u2010treated mice, some changes were observed in the size of the large pre\u2010B cell population and in the incidence of mitotic cells after vincristine administration, but the overall production rate of pre\u2010B cells did not differ from that in controls given normal rabbit serum. Pre\u2010B cell kinetics in WW and SI\/SI<jats:sup>d<\/jats:sup> mice also generally resembled those in homozygous controls. In contrast, after SRBC injection, there was an increase in the rate at which large pre\u2010B cells entered mitosis. Thus, the proliferation of c\u03bc<jats:sup>+<\/jats:sup>s\u03bc\u2010 bone marrow pre\u2010B cells shows no evidence of feedback control from the mature B lymphocyte pool, as indicated by lack of stimulation of pre\u2010B cell production in anti\u2010IgM\u2010treated mice, and is independent of the hemopoietic defects of WW or SI\/SI<jats:sup>d<\/jats:sup> mutants. On the other hand, the increased bone marrow pre\u2010B cell proliferation after SRBC injection demonstrates that the magnitude of B cell genesis in the bone marrow can be influenced by extrinsic agents and thus may be influenced by environmental stimuli.<\/jats:p>","DOI":"10.1002\/eji.1830150613","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T21:52:17Z","timestamp":1172699537000},"page":"599-605","source":"Crossref","is-referenced-by-count":35,"title":["Regulation of pre\u2010B cell proliferation in bone marrow: immunofluorescence stathmokinetic studies of cytoplasmic p chain\u2010bearing cells in anti\u2010IgM\u2010treated mice, hematologically deficient mutant mice and mice given sheep red blood cells"],"prefix":"10.1002","volume":"15","author":[{"given":"Davine","family":"Opstelten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis G.","family":"Osmond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(74)90233-0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"719","DOI":"10.4049\/jimmunol.113.3.719","volume":"113","author":"Ryser J. 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