{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:08:46Z","timestamp":1760645326358},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":7260,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Experiments were undertaken to localize in the lipopolysaccharide (LPS) the minimal structural determinants sufficient to initiate the signal leading to interleukin 1 (IL 1) secretion by human monocytes. Our results clearly demonstrated that this signal is triggered by structures present in the so\u2010called inner\u2010core region which chemically consists of 2\u2010keto\u20103\u2010deoxy\u2010w<jats:sc>D<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010octulosonic acid (KDo) and heptose in many LPS of gram\u2010negative bacteria. Thus, the isolated polysaccharide region of <jats:italic>Bordetella pertussis<\/jats:italic> endotoxin as well as fragments derived therefrom containing the reducing KDO unit were able to induce similar levels of IL1 induction as the native LPS. Similarly, the trisaccharide \u03b1\u2010<jats:sc>D<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010heptopyranosyl\u2010(1\u20133)\u2010\u03b1\u2010<jats:sc>D<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010heptopyranosyl\u2010(1\u20135)\u20103\u2010deoxy\u2010<jats:sc>D<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010octulosonic acid (hep\u2010hep\u2010KDO), representative for the inner\u2010core region of a large number of enterobacterial LPS, was a very potent IL 1 inducer. Neither KDO monosaccharide, nor the \u03b1\u2010(2\u20134)\u2010linked 3\u2010deoxy\u2010<jats:sc>D<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010octulosonic acid disaccharide isolated from <jats:italic>Salmonella<\/jats:italic> rough\u2010form LPS promoted the signal indicating that the minimal structure of endotoxin able to induce IL 1 secretion resides in the hep (1\u20135)\u2010KDO disaccharide.<\/jats:p>","DOI":"10.1002\/eji.1830160117","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T21:58:41Z","timestamp":1172699921000},"page":"87-91","source":"Crossref","is-referenced-by-count":51,"title":["Molecular requirement for interleukin 1 induction by lipopolysaccharide\u2010stimulated human monocytes: Involvement of the heptosyl\u20102\u2010keto 3\u2010deoxyoctulosonate region"],"prefix":"10.1002","volume":"16","author":[{"given":"Souad","family":"Lebbar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Marc","family":"Cavaillon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Caroff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annick","family":"Ledur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut","family":"Brade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Sarfati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Haeffner\u2010Cavaillon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"394","volume":"23","author":"Morrison D. C.","year":"1979","journal-title":"Adv. Immunol."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb08798.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830140202"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(80)90120-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.4049\/jimmunol.115.1.199","volume":"115","author":"Nowotny A.","year":"1975","journal-title":"J. Immunol."},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"847","DOI":"10.4049\/jimmunol.117.3.847","volume":"117","author":"Behling U. H.","year":"1976","journal-title":"J. Immunol."},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1128\/iai.42.1.250-256.1983","volume":"42","author":"Brade H.","year":"1983","journal-title":"Infect. Immun."},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"2294","DOI":"10.4049\/jimmunol.133.5.2294","volume":"133","author":"Williamson S. I.","year":"1984","journal-title":"J. Immunol."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(82)90001-6"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(84)90036-1"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1515\/znb-1952-0303"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1969.tb00601.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1975.tb04172.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1128\/iai.27.3.739-745.1980","volume":"27","author":"Ayme G.","year":"1980","journal-title":"Infect. Immun."},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1128\/jb.159.2.611-617.1984","volume":"159","author":"Moreau M.","year":"1984","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1984.tb08543.x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)84208-1"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1969.tb00781.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(83)88176-2"},{"key":"e_1_2_1_21_2","first-page":"205","volume":"55","author":"Hakomori S.","year":"1966","journal-title":"J. Biochem."},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1139\/o84-022"},{"key":"e_1_2_1_23_2","first-page":"1950","volume":"128","author":"Haeffner\u2010Cavaillon N.","year":"1982","journal-title":"Immunol."},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(85)90037-1"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(85)90047-1"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1975.tb02205.x"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(83)84020-8"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1968.tb00182.x"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb05871.x"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830160117","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830160117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T14:24:55Z","timestamp":1697811895000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830160117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/eji.1830160117"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830160117","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}