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Monoclonal antibodies to the LFA\u20101 \u03b1 and \u03b2 subunits, able to precipitate the LFA\u20101\u03b1, 180\u2010kDa chain, the p151 chain and \u03b2 94\u2010kDa chain (shared by both \u03b1 chains), were used in immunoprecipitation studies of patient and control phytohemagglutinin\u2010blasts. Neither of the \u03b1 chains nor the \u03b2 chain were found in precipitates obtained from <jats:sup>125<\/jats:sup>I\u2010surface\u2010labeled patient cells in contrast to controls. However, the precursor of the LFA\u20101 \u03b1 chain, a 170\u2010kDa polypeptide, was identified in lysates of biosynthetically labeled patients' cells. These results suggest that the defective membrane expression of the LFA\u20101 complex may be secondary to the absence of the mature \u03b2 chain.<\/jats:p>","DOI":"10.1002\/eji.1830160217","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T23:06:10Z","timestamp":1172703970000},"page":"205-208","source":"Crossref","is-referenced-by-count":19,"title":["Defective membrane expression of the LFA\u20101 complex may be secondary to the absence of the \u03b2 chain in a child with recurrent bacterial infection"],"prefix":"10.1002","volume":"16","author":[{"given":"Barbara","family":"Lisowska\u2010Grospierre","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Christine","family":"Bohler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Mawas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thymothy A.","family":"Sringer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Giscelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.23.7489"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.156.4.1000"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.4049\/jimmunol.131.3.1182","volume":"131","author":"Ware C. 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