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The hybridomas were selected for the expression of the Thy\u20101.2 antigen of th3 normal celldonor and for their ability t9produce interleukin 2 (IL 2) upon co\u2010culture with irradiated normal spleen cells. A set of these hybridomas is described in this communication. The hybridomas were then used to immunize rats and to generate monoclonal antibody\u2010producing B cell hybridomas. Most, if not all, of the immunizing hybridomas were derived from pre\u2010T cells as evidenced by the fact that they produce IL 2, and express some of the T cell markers (the Thy\u20101.2, Ly\u20101, Ly\u20102 or L3T4 antigens). The monoclonal antibodies were tested on a panel of pre\u2010T cell hybridomas and on normal cells obtained from spleen, lymph nodes, thymus and bone marrow. The testing was carried out by the microcytotoxicity assay and flow cytometric analysis. Three groups of antibodies could be distinguished. Some antibodies were broadly reactive, being positive with virtually all the clones in the pre\u2010T cell panel and with a substantial fraction of normal lymphoid cells. The identity of the antigens detected by these antibodies remains unknown but they do not seem to correspond to any of the known cell surface markers. Other antibodies reacted only with some of the pre\u2010T cell clones and did not react at all with normal lymphoid cells obtained from adult animals. Finally, other antibodies still reacted only with a minor subpopulation of thymocytes or of thymocytes and bone marrow cells, as well as some of the pre\u2010T cell clones; they did not react with spleen and lymph node cells. These antibodies might be specific for cells in the prethymic phase of the T cell differentiation pathway. They should prove useful for the identification of pre\u2010T cell markers and hence for the isolation of pre\u2010T cells and their functional analysis.<\/jats:p>","DOI":"10.1002\/eji.1830161113","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T22:42:22Z","timestamp":1172702542000},"page":"1391-1399","source":"Crossref","is-referenced-by-count":50,"title":["An attempt to produce \u201cpre\u2010T\u201d cell hybridomas and to identify their antigens"],"prefix":"10.1002","volume":"16","author":[{"given":"Yukoh","family":"Aihara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010J\u00f6rg","family":"B\u00fchring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiko","family":"Aihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Hematopoietic Stem Cells","author":"Golde D. 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