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We have previously described two unusual Lyt\u20102<jats:sup>+<\/jats:sup>L3T4<jats:sup>+<\/jats:sup> class II\u2010restricted T cell clones whose activation by antigen is inhibited by antibodies to L3T4 but not to Lyt\u20102 (B. Fazekas de St. Groth et al., <jats:italic>Proc. Natl. Acad. Sci. USA<\/jats:italic> 1986. <jats:italic>83<\/jats:italic>: 2594). The Lyt\u20102 immuno\u2010precipitated from one of these clones was indistinguishable from the molecule found on splenic T cells, as analyzed under reducing conditions on polyacrylamide gels, in two\u2010dimensional charge\/size separations and in peptide mapping. The molecule from the second clone showed slightly more extensive glycosylation but was within the range described for functional Lyt\u20102 on cytotoxic T cell lines. Lyt\u20102 mRNA from both clones showed no abnormalities on Northern analysis. Lyt\u20102 is normally expressed on thymocytes and peripheral T cells as a heterodimer disulfide bonded to the Lyt\u20103 glycopeptide, yet Lyt\u20103 could not be detected on the cell membranes of our clones; Lyt\u20102 existed as stable homodimers without Lyt\u20103. Thus Lyt\u20103 is not required structurally for the spontaneous expression of Lyt\u20102 on lymphoid cells.<\/jats:p>","DOI":"10.1002\/eji.1830161116","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T22:42:22Z","timestamp":1172702542000},"page":"1413-1417","source":"Crossref","is-referenced-by-count":8,"title":["Stable expression of Lyt\u20102 homodimers on L3T4<sup>+<\/sup>T cell clones"],"prefix":"10.1002","volume":"16","author":[{"given":"Pauline F.","family":"Gallagher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara Fazekas","family":"de St. Groth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques F. A. 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