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The <jats:italic>in vitro<\/jats:italic> IgE synthesis evoked by T cell clones was detectable between day 6 and 9 and peaked on day 12. Most clones maintained their ability to stimulate <jats:italic>in vitro<\/jats:italic> IgE synthesis in repeated assays over a 3\u2010month period. The induction of IgE synthesis by cloned T cells did not reflect alloantigen recognition on target B cells, since T cell clones induced IgE synthesis in B cells from all randomly selected donors tested, including autologous B cells.<\/jats:p><jats:p>Preincubation for 24 h with optimal stimulatory concentrations of anti\u2010CD 3 (OKT 3) monoclonal antibody or its addition through the entire culture period also enabled T cell clones to stimulate <jats:italic>de novo<\/jats:italic> IgE synthesis <jats:italic>in vitro<\/jats:italic> in normal B cells. Virtually all the T cell clones active on IgE synthesis induced the <jats:italic>in vitro<\/jats:italic> production of remarkable amounts of IgM and IgG as well.<\/jats:p><jats:p>These data indicate that several human T cell clones can induce normal B cells to synthesize immunoglobulin of different classes, including IgE, regardless of alloantigen recognition on target B cells or specificity for peculiar antigens. The activity of these clones was apparently mediated by triggering of the monomorphic molecular complex CD 3, immediately before or during the incubation of T cell clones with the target B cells.<\/jats:p>","DOI":"10.1002\/eji.1830161207","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T22:41:48Z","timestamp":1172702508000},"page":"1509-1514","source":"Crossref","is-referenced-by-count":24,"title":["Human T cell clones can induce <i>in vitro<\/i> IgE synthesis in normal B cells regardless of alloantigen recognition or specificity for peculiar antigens"],"prefix":"10.1002","volume":"16","author":[{"given":"Gianfranco","family":"Del Prete","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Maggi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donatella","family":"Macchia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Tiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Parronchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Ricci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Romagnani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(83)90080-4"},{"key":"e_1_2_1_3_2","first-page":"465","volume-title":"Progress in Immunology V","author":"Katz D. 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