{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:43:38Z","timestamp":1698212618437},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":7265,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antibodies against the T44 surface molecule have been shown to activate human T cells to produce interleukin 2. The role of Ca<jats:sup>2+<\/jats:sup> in the triggering of the interleukin 2\u2010producing Jurkat T cell line by anti\u2010T44 monoclonal antibody has been investigated. We show that activation is initiated by an increase in the concentration of free cytoplasmic calcium ions [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. Subsequently, we have investigated the mechanism by which perturbation of T44 molecules induces increases of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in Jurkat cells. We show that the anti\u2010T44\u2010mediated increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> can occur only in presence of extracellular Ca<jats:sup>2+<\/jats:sup>, since no increment is detectable when extracellular Ca<jats:sup>2+<\/jats:sup> is depleted by EGTA. Thus, it appears that perturbation of T44 molecules, unlike that of T3\u2010Ti antigen receptor complex, fails to mobilize Ca<jats:sup>2+<\/jats:sup> from intracellular stores. As inositol triphosphate is considered the putative mobilizer of Ca<jats:sup>2+<\/jats:sup> from internal stores, we measured the levels of inositol triphosphate and of the other inositol phosphate compounds in Jurkat cells after stimulation with anti\u2010T44 antibodies. In contrast to the stimulation via the T3\u2010Ti antigen receptor complex, stimulation via T44 molecule does not induce increments of all three inositol phosphates. Taken together, these data indicate that stimulation mediated by the T44 molecule proceeds via a mechanism independent from the typical inositol lipid metabolism which does not involve mobilization of Ca<jats:sup>2+<\/jats:sup> from internal stores.<\/jats:p>","DOI":"10.1002\/eji.1830161228","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T22:41:48Z","timestamp":1172702508000},"page":"1639-1642","source":"Crossref","is-referenced-by-count":19,"title":["Signal transducing mechanisms involved in human T cell activation via surface T44 molecules. Comparison with signals transduced via the T cell receptor complex"],"prefix":"10.1002","volume":"16","author":[{"given":"Giuseppe","family":"Pantaleo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Olive","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Poggi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Moretta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Moretta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.3.988"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.124.6.2708"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90039-4"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.6.1513"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.3.823"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830120115"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2935936"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(75)90017-9"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.54.070185.001225"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2200345"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/306067a0"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/308693a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.13.4169"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2190661"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.134.2.663"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.3.446"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910360219"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01561573"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"3077","DOI":"10.1016\/S0021-9258(17)43262-5","volume":"259","author":"Thomas A. 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