{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:28:55Z","timestamp":1789644535596,"version":"4.0.0"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":6895,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The defective membrane expression of the adhesive protein family (LFA\u20101, Mo1 and p 150, 93) on leukocytes from certain patients with recurrent bacterial infections was shown to be secondary to the absence of synthesis of mature \u03b2 chain that is common to all three antigens (Springer et al., 1984, Lisowska\u2010Grospierre et al. 1986). In all patients, studies of \u03b2\u2010chain biosynthesis that lead to this conclusion were performed using the monoclonal anti\u2010\u03b2 chain antibody to isolate the \u03b2 subunit. Since this antibody detects the mature form of \u03b2 chainonly, the potential presence of a precursor or of an abnormal \u03b2 chain in the patient's cells could not be tested. the availability of the polyclonal antibody to the purified \u03b2 subunit allowed us t re\u2010examine the biosynthesis of the LFA\u20101 subunits in 3 affected children. In al 3 patients, the absence of membrane expression of the LFA\u20101, CR3 and p150,95 proteins was confirmed. The LFA\u20101 \u03b1\u2010chain precursor of 170 kDa was detected in the lysates of PHA blasts of two children, but was not detected in the third. The \u03b2\u2010chain precursor of 85 kDa was isolated by the polyclonal anti\u2010\u03b2 chain antiserum from the cytoplasm of phytohemagglutinin and Epstein\u2010Barr virus\u2010induced blasts of one patient. The same antibody precipitated some peptides of smaller mol. wt. from the cell lysates of 2 other patients. These results suggest that in this disorder the membrane nonexpression of the adhesive proteins is probably due to the structural abnormality of \u03b2 chain which, although synthesized, is rapidly degradated.<\/jats:p>","DOI":"10.1002\/eji.1830170318","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:30:33Z","timestamp":1172709033000},"page":"417-419","source":"Crossref","is-referenced-by-count":28,"title":["LFA\u20101 \u03b2\u2010chain synthesis and degradation in patients with leukocyte\u2010adhesive proteins deficiency"],"prefix":"10.1002","volume":"17","author":[{"given":"Marie\u2010Ther\u00e8se","family":"Dimanche","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7","family":"Le Deist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Amin","family":"Arnaout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Griscelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Lisowska\u2010Grospierre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.124.4.1943"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.154.5.1517"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.6.1785"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.2.586"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830150714"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111186"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111451"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111539"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.6.1901"},{"key":"e_1_2_1_11_2","first-page":"91","volume-title":"Progress in Immunodeficiency Research and Therapy","author":"Lisowska\u2010Grospierre B.","year":"1984"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112251"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/152.4.668"},{"key":"e_1_2_1_14_2","first-page":"2664","volume":"44","author":"Arnaout M. A.","year":"1985","journal-title":"Fed. Proc."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830160217"},{"key":"e_1_2_1_16_2","unstructured":"Dana N. Clayton L. K. Tennen D. G. Pierce M. W. Lachmann P. J. Law S. A. K.andArnaout M. A. J. Clin. Invest. in press."},{"key":"e_1_2_1_17_2","first-page":"256","volume-title":"6th International Congress of Immunology","author":"Te Heroo Pat","year":"1986"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)81148-6"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830170318","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830170318","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T05:09:27Z","timestamp":1697864967000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830170318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,1]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1987,1]]}},"alternative-id":["10.1002\/eji.1830170318"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830170318","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,1]]}}}