{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:44:58Z","timestamp":1762271098569},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":6895,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The results described here provided an example of a human IgM monoclonal antibody against a tumor\u2010associated glycolipid and of the unusual properties of its corresponding immunotoxin (IT). The monoclonal antibody referred to as 38\u201013 has been previously described and reacted with the globotriaosylceramide [Gb<jats:sub>3<\/jats:sub>: Gal(\u03b11\u21924)\u2010Gal(\u03b21\u21924)\u2010Glc(\u03b21\u21921)ceramide] specifically expressed on surface membrane of Burkitt's lymphoma (BL) cells. An immunotoxin (38\u201013 IT) combined with the pokeweed antiviral protein (PAP) toxin via S\u2010S bridges showed paradoxically a lower cytotoxic effect in BL Ramos cells than in non\u2010BL cells such as leukemic mouse L1210 cells, while these cells appeared not to be involved by flow cytometric analysis and complement\u2010dependent cytotoxicity. Consequently, the inhibitory effect of selective galactose analogs on the binding and the cytotoxicity of 38\u201013 antibody conjugated or not to PAP toxin was compared on BL and non\u2010BL cells. Only the galactose blocked in \u03b1 configuration provided a fine inhibition of 38\u201013 binding on BL Ramos cells and both \u03b1 and \u03b2\u2010galactose allowed us to establish a clear distinction between the pathway entry of 38\u201013 IT in BL and non\u2010BL cells; (a) in close correlation with the 38\u201013 binding specificity the 38\u201013 IT cytotoxic effect in Ramos BL cells could also be prevented by \u03b1\u2010Gal only, suggesting that this toxic action is probably mediated through the IT binding to Gb<jats:sub>3<\/jats:sub> antigenic sites. (b) In contrast, on apparently irrelevant L1210 cells, 38\u201013 IT showed a cytotoxic effect which was inhibited preferentially by lactose (Gal in \u03b2 configuration). It was discussed that IT binding alone to either antigenic sites which are inhibited by the hapten \u03b1\u2010Gal, or nonspecific sites which can compete with the hapten \u03b2\u2010Gal is unable to induce efficient killing of cells. But cooperation of both bindings might give an attractive explanation of IT cytotoxic effect. It was concluded that the unexpected activity of 38\u201013 IT in non\u2010BL cells probably could be mediated through an active macromolecular transport process which could implicate a \u03b2\u2010galactoside\u2010binding protein (lectin).<\/jats:p>","DOI":"10.1002\/eji.1830170404","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:47:54Z","timestamp":1172710074000},"page":"459-464","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of inhibitory effect of galactose analogs on the binding and cytotoxicity of an anti\u2010globotriaosylceramide monoclonal antibody coupled or not coupled to pokeweed antiviral protein"],"prefix":"10.1002","volume":"17","author":[{"given":"Simone","family":"Junqua","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Wils","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohar","family":"Mishal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Bernard","family":"Le Pecq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(81)90050-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6218613"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(85)12018-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910300410"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90366-9"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/294171a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1016\/S0021-9258(17)43579-4","volume":"259","author":"Raso V.","year":"1984","journal-title":"J. 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