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Its biochemical characterization was undertaken, using trypsin treatment of Tg, followed by gel filtration, affinity chromatography, sodium dodecyl sulfate\u2010poly\u2010acrylamide gel electrophoresis and high pressure liquid chromatography analysis. Immunoreactivity tested by immunoblotting with appropriate monoclonal anti\u2010Tg antibody allowed the detection of one peptide fragment of 5\u201310 kDa, after limited proteolysis with trypsin. We demonstrated that this peptide reacts exclusively with monoclonal anti\u2010Tg antibodies which block <jats:italic>in vitro<\/jats:italic> proliferative T cell response; moreover, the 5\u201310\u2010kDa tryptic fragments from porcine Tg were able to induce thyroiditis when injected into normal CBA mice, while porcine Tg depleted from these fragments was not able to do so.<\/jats:p>","DOI":"10.1002\/eji.1830170617","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:40:02Z","timestamp":1172709602000},"page":"843-848","source":"Crossref","is-referenced-by-count":30,"title":["Experimental autoimmune thyroiditis induced by a 5\u201310\u2010kDa tryptic fragment from porcine thyroglobulin"],"prefix":"10.1002","volume":"17","author":[{"given":"Jean","family":"Salamero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Jacques","family":"Remy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Michel\u2010Bechet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeannine","family":"Charreire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/316647a0"},{"key":"e_1_2_1_3_2","first-page":"54","volume":"1","author":"Silverman D.","year":"1978","journal-title":"J. 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