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Immunol.<\/jats:italic> 1985. <jats:italic>15:<\/jats:italic> 611) that interleukin 2 (IL 2) in this material is able to induce T cell\u2010dependent IgM secretion in normal B cells. Evidence was obtained for the presence of another factor distinct from IL 2 that could replace T cells in the induction of B cell differentiation. We have analyzed this factor with the use of a neoplastic B cell population of prolymphocytic origin that was functionally nonresponsive to IL 2. T cell\u2010replacing factor (TRF)\u2010like activity and IL 2 could be separated by ion\u2010exchange chromatography, although a small amount of IL 2 was recovered in the TRF fractions. This small amount of IL 2 was found to be crucial for the observed TRF activity. Moreover, a substantial amount of monomeric Con A was detected in the TRF preparation. Our studies show that Con A in the presence of IL 2 can act as a potent inducer of helper function in lower numbers of T cells for normal and neoplastic B cells. Functional assays for T cell contamination in B cell suspensions are therefore of limited value because they are determined by the efficiency of the stimulating signal. Particularly in those B cell factor preparations, obtained from mitogen\u2010activated T cells with an obligatory or unidentified role of IL 2, the possible effect of a contaminating mitogen must be considered.<\/jats:p>","DOI":"10.1002\/eji.1830170811","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:55:42Z","timestamp":1172710542000},"page":"1145-1150","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of T cell\u2010replacing factor\u2010like activity: potent induction of T helper activity for human B cells by residual concanavalin A and interleukin 2"],"prefix":"10.1002","volume":"17","author":[{"given":"Robert W.","family":"Sauerwein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter G. 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