{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T21:40:38Z","timestamp":1698010838125},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":6535,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1988,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel anti\u2010B cell monoclonal antibody UCHB1 is described which detects an antigen present on a selected subpopulation of both normal and leukemic B cells. Approximately 20% of normal tonsil and peripheral blood (PB) B cells are UCHB 1<jats:sup>+<\/jats:sup> and the majority of B cells from all cases of prolymphocytic leukemia (PLL) tested, together with a low, variable percentage of PB B cells from non\u2010Hodgkin's lymphoma patients with centroblastic centrocytic leukemia also express this antigen.<\/jats:p><jats:p>Chronic lymphocytic leukemia and hairy cell leukemia B cells, pre\u2010B acute lymphoblastic leukemia and Epstein\u2010Barr virus transformed cell lines all lack UCHB1 positivity as do all non\u2010B lineage leukocytes and cell lines so far tested.<\/jats:p><jats:p>In tonsil sections UCHB 1 staining is almost completely confined to surface IgM<jats:sup>+<\/jats:sup> mantle zone lymphocytes and follicular dendritic cells, but not B cells, within the germinal centers. UCHB 1 can induce a rise in the level of intracellular free calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in PLL B cells and low concentrations of monoclonal antibody can result in the entry of these cells into cell cycle in the absence of additional factors. The proliferative effect of UCHB 1 is greatly enhanced in the presence of <jats:italic>Staphylococcus aureus<\/jats:italic> Cowan and, to a lesser extent, phorbol ester. A similar costimulatory effect is exerted on a proportion of normal tonsil B cells although here, despite inducing a rise in [Ca<jats:sup>2+<\/jats:sup>]i, UCHB1 alone does not cause cells to proliferate. UCHB 1 may well prove to be a useful antibody both to distinguish between different B cell leukemias and to study the phenotype and function of leukemic and normal B cell subpopulations.<\/jats:p>","DOI":"10.1002\/eji.1830180111","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:50:13Z","timestamp":1172710213000},"page":"67-76","source":"Crossref","is-referenced-by-count":6,"title":["A new antigen identified by the monoclonal antibody UCHB1 delivers a costimulatory signal to a subset of human B cells"],"prefix":"10.1002","volume":"18","author":[{"given":"Richard J.","family":"Armitage","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah J.","family":"Rowe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C. 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