{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T05:00:31Z","timestamp":1757566831413},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":6383,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1988,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To identify the molecular localization and specificity of <jats:italic>Mycobacterium leprae<\/jats:italic> antigenic determinants inducing T cell activation, we studied the reactivity of <jats:italic>M. leprae\u2010<\/jats:italic>reactive T cell clones from two tuberculoid leprosy patients towards a battery of different mycobacterial strains and purified mycobacterial antigens. Of the 38 T cell clones tested 8 appeared to be <jats:italic>M. leprae<\/jats:italic> specific (specificity A), another 8 were cross\u2010reactive with at least one of the three mycobacterial strains, <jats:italic>M. lepraemurium, M. vaccae<\/jats:italic> and <jats:italic>M. scrofulaceum<\/jats:italic> (specificity B), 5 were reactive with most but not all strains (specificity C) and the remaining 18 were reactive with all 17 mycobacterial strains tested (specificity D), but not with nonmycobacterial antigens. All T cell clones were tested with the 36K and 65K antigen isolated from <jats:italic>M. leprae<\/jats:italic>, and with the <jats:italic>M. leprae<\/jats:italic> and <jats:italic>M. bovis<\/jats:italic> BCG 65K proteins produced in <jats:italic>E. coli<\/jats:italic> by recombinant DNA. Four T cell clones appeared to recognize epitopes on the 36K antigen, nine T cell clones recognized the 65K antigen. These 2 <jats:italic>M. leprae<\/jats:italic> antigens, 36K and 65K, thus seem to contain major T cell epitopes. At least 3 different epitopes could be defined on the 36K antigen of which one is <jats:italic>M. leprae<\/jats:italic> specific, one of specificity B and one of specificity C. Two distinct epitopes were discerned on the 65K antigen of which one is <jats:italic>M. leprae<\/jats:italic> specific and one of specificity D. The <jats:italic>M. leprae<\/jats:italic>\u2010specific epitopes on the 36K and 65K antigen may help in the development of a specific serodiagnostic and skin test.<\/jats:p>","DOI":"10.1002\/eji.1830180604","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T05:26:21Z","timestamp":1172726781000},"page":"849-854","source":"Crossref","is-referenced-by-count":24,"title":["T cell epitopes on the 36K and 65K Mycobacterium leprae antigens defined by human T cell clones"],"prefix":"10.1002","volume":"18","author":[{"given":"Wim C. 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