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We demonstrate that TSP\u20101 mRNA is only expressed in activated T lymphocytes and is absent from all mouse tissues tested including those containing resting mature T lymphocytes. Expression of the TSP\u20101 gene was observed in T lymphoctes <jats:italic>in vitro<\/jats:italic> in response to either phorbolester (phorbol 12\u2010myristate 13\u2010acetate), Ca<jats:sup>2+<\/jats:sup> ionophore (A23187), lectin or alloantigen. In general, TSP\u20101 mRNA appeared and peaked later compared to interleukin 2 transcripts. Furthermore, TSP\u20101 mRNA was inducible <jats:italic>in vitro<\/jats:italic> in both Ly\u20102<jats:sup>+<\/jats:sup> and L3T4<jats:sup>+<\/jats:sup> lymphocyte populations treated with alloantigen and\/or lectin. The transcription of the TSP\u20101 gene was always accompanied by the expression of proteinase activity. High expression of TSP\u20101 transcripts was also observed in <jats:italic>in vivo<\/jats:italic> derived T effector cells specific for lymphocytic choriomeningitis virus. However, TSP\u20101 mRNA was predominantly associated with virus\u2010specific Ly\u20102<jats:sup>+<\/jats:sup> T cells and correlated with their proteinase and cytolytic activities. The data suggest that TSP\u20101 gene transcription is a useful marker to characterize T effector cells <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo.<\/jats:italic><\/jats:p>","DOI":"10.1002\/eji.1830180605","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T05:26:21Z","timestamp":1172726781000},"page":"855-861","source":"Crossref","is-referenced-by-count":13,"title":["Induction of T cell serine proteinase 1 (TSP\u20101)\u2010specific mRNA in mouse T lymphocytes"],"prefix":"10.1002","volume":"18","author":[{"given":"Hans\u2010Georg","family":"Simon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uli","family":"Fruth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Eckerskorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich","family":"Lottspeich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael D.","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabi","family":"Nerz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus M.","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.03.040185.001025"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(87)90844-9"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.136.12.4644"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04638.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04401.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/322740a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90441-1"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170506"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"566","DOI":"10.4049\/jimmunol.138.2.566","volume":"138","author":"Takayama H.","year":"1987","journal-title":"J. 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