{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T21:55:42Z","timestamp":1757541342859},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":6383,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1988,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Functions of T cells derived from single progenitor cells were investigated. B10. Thy\u20101.1 recipient mice were either whole body\u2010irradiated and marrow reconstituted or thymus\u2010shielded, irradiated and marrow reconstituted, and limited numbers (3 \u00d7 10<jats:sup>3<\/jats:sup> or 6 \u00d7 10<jats:sup>3<\/jats:sup>) of a 1:1 mixture of bone marrow cells from C57BL\/6 and B6.Lyt\u20102.1 mice were transferred intrathymically (i.t.). Donor\u2010type (Thy\u20101.2<jats:sup>+<\/jats:sup>) cells of the thymus of a small portion of recipients which expressed the phenotype of either Ly\u20102.2 or Ly\u20102.1 but not both were regarded to be a clone of T cells derived from a single progenitor cell, and such clones were assayed for polyclonal helper (T<jats:sub>h<\/jats:sub>) and polyclonal cytolytic (CTL) activities as well as alloantigen\u2010specific proliferative (mixed lymphocyte reaction; MLR) and CTL activities. Clones taken 4 weeks after transfer (4\u2010week\u2010old clones) which were generated in the thymus of whole body\u2010irradiated recipients showed polyclonal CTL but not polyclonal T<jats:sub>h<\/jats:sub> activity, whereas 4\u2010week\u2010old clones generated in the thymus of thymus\u2010shielded recipients showed both polyclonal CTL and T<jats:sub>h<\/jats:sub> activities. Similarly, 4\u2010week\u2010old clones generated in whole body\u2010irradiated recipients responded with CTL to alloantigens when induced in the presence of T cell growth factors but not with MLR, whereas 4\u2010week\u2010old clones generated in thymus\u2010shielded recipients showed both MLR and CTL to alloantigens. Repertoire difersifi\u2010cation of 4\u2010week\u2010old clones, however, was incomplete, since clones generated in whole body\u2010irradiated recipient did not necessarily respond with CTL to all alloantigens examined, and those generated in thymus\u2010shielded recipients did not necessarily respond with MLR to all the antigens. On the other hand, T cell clones were shown to fully mature by 7 weeks after transfer in terms of cell function as well as repertoire diversification.<\/jats:p>","DOI":"10.1002\/eji.1830180610","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T05:26:21Z","timestamp":1172726781000},"page":"897-903","source":"Crossref","is-referenced-by-count":5,"title":["Functional differentiation and repertoire diversification of T cells derived from single progenitor cells"],"prefix":"10.1002","volume":"18","author":[{"given":"Tatsuo","family":"Kina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Amagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Ichi","family":"Nishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Ichi","family":"Araya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimoto","family":"Katsura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830010102"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000313401"},{"key":"e_1_2_1_4_2","first-page":"1","volume":"29","author":"Owen J. 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