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All such specificities are recovered as activated blasts after density gradient fractionation, the \u201csmall lymphocyte\u201d pool being depleted of anti\u2010BMRC reactivities. In contrast, the spleens of athymic (nude) mice contain undetectably low frequencies of these specificities in either lymphocyte compartment. Transfer of relatively low numbers of normal syngeneic splenic T lymphocytes to adult nude mice restores the high frequency of anti\u2010BMRC clonal precursor B cells, again in the activated, but not in the resting spleen cell fractions. Large total T cells are more than tenfold better than resting T cells in reconstitution potential, as are enriched CD4<jats:sup>+<\/jats:sup> as compared to CD8<jats:sup>+<\/jats:sup> cells which are practically devoid of activity in this respect. These results apply exclusively to B cells at a differentiative stage that allows for extensive clonal expansion, since there is a marked difference between the frequency of clonal precursors determined by limiting dilution analysis and the frequency of Ig\u2010secreting plaque\u2010forming cells of the same specificity, and induced by the same mitogen in short\u2010term cultures. The implications of these findings for the physiology of autoreactivity and repertoire selection in the compartment of perinatal B cells are discussed.<\/jats:p>","DOI":"10.1002\/eji.1830181022","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T05:07:48Z","timestamp":1172725668000},"page":"1615-1622","source":"Crossref","is-referenced-by-count":50,"title":["T cell dependence of the \u201cnatural\u201d autoreactive B cell activation in the spleen of normal mice"],"prefix":"10.1002","volume":"18","author":[{"given":"Fan\u00e7ois","family":"Huetz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva\u2010Lotta","family":"Sciard\u2010Larsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis Portnoi","family":"And","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f6nio","family":"Coutinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.140.5.1189"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.4049\/jimmunol.119.6.2151","volume":"119","author":"Izui S.","year":"1977","journal-title":"J. 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