{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:05:51Z","timestamp":1762067151446},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":6230,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1988,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A monoclonal antibody (mAb) G\u20105\u20102 was isolated which binds to transformed as well as normal cells of the B lineage but not to cells of the T cell, myeloid lineages nor to fibroblasts. mAb G\u20105\u20102 reacts with pre\u2010B and plasma cell\u2010transformed lines, and it preferentially recognizes normal pre\u2010B cells from fetal liver and bone marrow as well as plasma cells from spleen of mice. G\u20105\u20102<jats:sup>+<\/jats:sup> fetal liver cells isolated by cell sorter express mRNA for \u03bc heavy chain Ig gene and generate <jats:italic>in vitro<\/jats:italic> antibody\u2010producing cells when co\u2010cultured with lipopolysaccharide and rat thymocyte filler cells. During development the frequency and staining intensity of G\u20105\u20102<jats:sup>+<\/jats:sup> cells in fetal liver from normal mice increases from 1% G\u20105\u20102<jats:sup>+<\/jats:sup> cells at day 14 to\u223c7% positive cells at day 18 of gestation. Several strains or normal mice contain comparable numbers of G\u20105\u20102<jats:sup>+<\/jats:sup> cells as well as B\u2010220<jats:sup>+<\/jats:sup> and BP\u20101<jats:sup>+<\/jats:sup> B cell precursors in the fetal liver. Mice carrying the <jats:italic>xid<\/jats:italic> mutation have 3\u20104\u2010fold less G\u20105\u20102<jats:sup>+<\/jats:sup> as well as B\u2010220<jats:sup>+<\/jats:sup> and BP\u20101<jats:sup>+<\/jats:sup> cells in the fetal liver, suggesting that the effects of the <jats:italic>xid<\/jats:italic> mutation may be manifested from early stages of B cell development. Fetal liver cells from mice carrying the <jats:italic>scid<\/jats:italic> mutation were found to contain normal numbers of G\u20105\u20102<jats:sup>+<\/jats:sup> as well as B\u2010220<jats:sup>+<\/jats:sup> and BP\u20101<jats:sup>+<\/jats:sup> pre\u2010B cells. These results indicate that differentiation from progenitors to pre\u2010B cells in <jats:italic>scid<\/jats:italic> mice may occur normally; the <jats:italic>scid<\/jats:italic> mutation would thus appear to affect the process of rearrangement and expression of the Ig genes in the developing pre\u2010B cells. mAb G\u20105\u20102 precipitates a 76\u2010kDa glycoprotein from surface\u2010radiolabeled pre\u2010B cells and plasma cells. Taken together, these results indicate that G\u20105\u20102 mAb recognizes a novel B cell lineage\u2010specific surface molecule called PB76 which is preferentially expressed by pre\u2010B cells and plasma cells.<\/jats:p>","DOI":"10.1002\/eji.1830181123","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T01:04:54Z","timestamp":1172711094000},"page":"1803-1810","source":"Crossref","is-referenced-by-count":12,"title":["PB76: a novel surface glycoprotein preferentially expressed on mouse pre\u2010B cells and plasma cells detected by the monoclonal antibody G\u201052"],"prefix":"10.1002","volume":"18","author":[{"given":"Andreas","family":"Strasser","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.6.1675"},{"key":"e_1_2_1_3_2","unstructured":"Strasser A. Ph. 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