{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T08:59:34Z","timestamp":1770713974292,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":6169,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An <jats:italic>in vitro<\/jats:italic> system for transforming immature lymphoid cells present in the thymus at early development has been established. By phenotype analysis of the transformants obtained, we observed that B cell precursors, susceptible to Abelson murine leukemia virus (A\u2010MuLV)\u2010 or Harvey murine sarcoma virus (H\u2010MuSV)\u2010induced lymphogenesis, were present at high frequency in the fetal thymus of BALB\/c mice. These precursors recolonized alymphoid thymus lobes <jats:italic>in vitro<\/jats:italic>, as do T cell precursors. It was further observed that B precursors in the fetal liver were also capable of recolonizing alymphoid thymus lobes and were stored in a thymic environment. These results suggest that stroma cells of the fetal thymus may possess the capacity to support the growth of B precursors. On the other hand, B cell precursors sensitive to the viral transformation were undetectable in the fetal thymus of C57BL\/6, although immunohistochemical analysis suggested their presence. However, in the fetal liver of the same strain, B precursors recolonizing alymphoid thymus <jats:italic>in vitro<\/jats:italic> were sensitive to the viral transformation. Based on these results, we will discuss both the role and fate of thymic B precursors. In addition, we also obtained T cell lymphomas at different stages of differentiation from the fetal thymus of C57BL\/6 infected with A\u2010MuLV or H\u2010MuSV. These data indicate the usefulness of our system in establishing cell lines derived from intrathymic lymphogenesis at early development.<\/jats:p>","DOI":"10.1002\/eji.1830190116","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T09:19:51Z","timestamp":1172740791000},"page":"97-104","source":"Crossref","is-referenced-by-count":20,"title":["B cell precursors are present in the thymus during early development"],"prefix":"10.1002","volume":"19","author":[{"given":"Hiroshi","family":"Kimoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuji","family":"Shirasawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Taniguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshitada","family":"Takemori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.126.4.715"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.129.2.431"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.153.2.280"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830120710"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830141217"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/317811a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/329336a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830171107"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.05.040187.001545"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.4049\/jimmunol.127.6.2262","volume":"127","author":"Kincade P. 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