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More than 50% of Ly\u20102(CD8) cells but only low numbers of Ly\u20102<jats:sup>\u2010<\/jats:sup>(CD8<jats:sup>\u2010<\/jats:sup>) cells, previously enriched by flow cytofluorometry, contained large amounts of cytoplasmic granules which stained both with MTSP\u20101\u2010specific mAb and the esterase substrate N\u2010\u03b1\u2010benzyloxycarbonyl\u2010<jats:sc>L<\/jats:sc>\u2010lysine thiobenzyl ester. These data demonstrate that <jats:italic>in vivo<\/jats:italic> generated LCM virus\u2010induced cytolytic T lymphocytes develop cytoplasmic storage granules containing MTSP\u20101 and suggest that the mechanism of granule exocytosis is operative <jats:italic>in vivo<\/jats:italic>; possibly MTSP\u20101 is one effector molecule participating in the Ly\u20102(CD8) T lymphocyte\u2010mediated control of virus infection.<\/jats:p>","DOI":"10.1002\/eji.1830190124","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T09:19:51Z","timestamp":1172740791000},"page":"151-156","source":"Crossref","is-referenced-by-count":28,"title":["Expression of cytoplasmic granules with T cell\u2010associated serine proteinase\u20101 activity in Ly\u20102(CD8) T lymphocytes responding to lymphocytic choriomeningitis virus <i>in vivo<\/i>"],"prefix":"10.1002","volume":"19","author":[{"given":"Michael D.","family":"Kramer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uli","family":"Fruth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Georg","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus M.","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.117.5_Part_1.1495"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.154.2.225"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-64-2-443"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.3.814"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"698","DOI":"10.4049\/jimmunol.136.2.698","volume":"136","author":"Byrne J. 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