{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:26:52Z","timestamp":1770751612868,"version":"3.50.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:00:00Z","timestamp":1133827200000},"content-version":"vor","delay-in-days":6152,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1989,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Follicular B cell lymphomas of SJL mice [reticulum cell sarcoma (RCS)] are dependent on syngeneic Ly\u20101,2<jats:sup>\u2010<\/jats:sup> T cells for their growth. These T cells produce a number of lymphokines in response to stimulation with \u03b3\u2010irradiated RCS cells including interleukin (IL) 2, interferon\u2010\u03b3 (IFN\u2010\u03b3), IL 4 and IL 5, some of which may be required for growth of the tumor. Previous studies have shown that an RCS cell line, cRCS\u2010X, can be maintained <jats:italic>in vitro<\/jats:italic> indefinitely, if the cultures are supplemented with Y\u2010irradiated lymph node (LN) cells or with a preparation of human B cell growth factor (BCGF). In the present studies, the growth requirements of this cell line were analyzed in more detail in short\u2010term assays of both [<jats:sup>3<\/jats:sup>H]thymidine incorporation and colony formation in agarose. Recombinant murine IL 5 caused dose\u2010dependent proliferation of cRCS\u2010X cells similar to that induced with BCGF. The level of colony formation by cRCS\u2010X cells induced by optimal concentrations of BCGF was not increased further by the addition of IL 5, suggesting that the two factors act via a common mechanism. IL 1 and IFN\u2010y each enhanced cRCS\u2010X proliferation induced by BCGF or IL 5 in both assays. The effects of IL 1 plus BCGF, IFN\u2010\u03b3 plus BCGF, and IL 1 plus IL 5 were clearly synergistic. Preincubation of cRCS\u2010X cells with IL 1 enhanced their ability to proliferate in response to BCGF or IL 5 in [<jats:sup>3<\/jats:sup>H]thymidine incorporation assays, but the reverse sequence of cytokine addition showed no effect of IL 1. No such effect was seen with IFN\u2010gM. Indeed, IL 1 and IFN\u2010gM appeared to affect BCGF\u2010induced cRCS\u2010X growth by different mechanisms and their combined effects were greater than that of IL 1 or IFN\u2010gM added separately. None of the other cytokines studied, including IL 2, IL 3, IL 4, IL 6, tumor necrosis factor\u2010\u03b3, granulocyte monocyte\u2010colony\u2010stimulating factor or transforming growth factor\u2010\u03b2, had any detectable effect on cRCS\u2010X cells, either alone or in combination with BCGF or IL 5.<\/jats:p><jats:p>Like IL 5, SJL \u03b3\u2010irradiated LN cells induced cRCS\u2010X colony\u2010forming units (CPU) in a dose\u2010dependent manner. IL 1, or the combination of IL 1 plus IFN\u2010\u03b3, clearly synergized with LN cells in the induction of cRCS\u2010X CFU, suggesting that LN cells contribute IL 5. The level of CFU induced by an optimal dose of BCGF was enhanced further in the presence of LN cells and IL 1. However, this was not observed when both IL 1 and IFN\u2010\u03b3 were added to the medium, in which case the level of CFU induced by an optimal dose of BCGF was not further enhanced by the addition of an optimal dose of \u03b3\u2010irradiated LN cells. In combination with previous observations, these data support the hypothesis that RCS growth <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> is dependent on and promoted by IL 5 and IFN\u2010\u03b3 produced by syngeneic RCS\u2010specific helper T cells, and IL 1 produced by macrophages.<\/jats:p>","DOI":"10.1002\/eji.1830190222","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T10:41:59Z","timestamp":1172745719000},"page":"365-371","source":"Crossref","is-referenced-by-count":27,"title":["Characterization and growth factor requirements of SJL lymphomas. II. Interleukin 5 dependence of the <i>in vitro<\/i> cell line, cRCS\u2010X, and influence of other cytokines"],"prefix":"10.1002","volume":"19","author":[{"given":"Jennifer L.","family":"Lasky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Jeanette Thorbecke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,6]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"771","volume":"40","author":"Dunn T. B.","year":"1968","journal-title":"J. Natl. Cancer Inst."},{"key":"e_1_2_1_3_2","first-page":"46","volume":"4","author":"Murphy E. D.","year":"1963","journal-title":"Proc. Am. Assoc. Cancer Res."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830188609056610"},{"key":"e_1_2_1_5_2","first-page":"237","volume":"113","author":"Pattengale P. K.","year":"1983","journal-title":"Am. J. 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