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Fixation with paraformaldehyde, an inhibitor of antigen processing, did not remove TSST\u20101\u2010binding activity of the transfectants. Binding of <jats:sup>125<\/jats:sup>I\u2010labeled TSST\u20101 to the transfectants was reduced by an anti\u2010DR monoclonal antibody. Sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis revealed the presence of a single band with TSST\u20101\u2010binding activity and the same migration pattern as DR heterodimers. TSST\u20101\u2010induced T cell responses, proliferation and interleukin 2 (IL2) production were observed in the presence of the transfectants but not in the presence of control L cells, while concanavalin A\u2010induced IL2 production was observed in the presence of either the transfectants or control L cells. Presence of an anti\u2010DR monoclonal antibody inhibited the TSST\u20101\u2010induced responses. Paraformaldehyde\u2010fixed Daudi cells were effective in supporting TSST\u20101\u2010induced IL2 production by T cells. These results indicate that HLA class II molecules directly bind intact TSST\u20101 and perform an essential role as the TSST\u20101\u2010binding structures on accessory cells in T cell activation by the toxin.<\/jats:p>","DOI":"10.1002\/eji.1830191007","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T08:33:43Z","timestamp":1172738023000},"page":"1803-1809","source":"Crossref","is-referenced-by-count":33,"title":["Activation of human t cells by toxic shock syndrome toxin\u20101: the toxin\u2010binding structures expressed on human lymphoid cells acting as accessory cells are hla class ii molecules"],"prefix":"10.1002","volume":"19","author":[{"given":"Takehiko","family":"Uchiyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken'Ichi","family":"Imanishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minako","family":"Araake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Jie","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Fujikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Igarashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehito","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiya","family":"Obata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noboru","family":"Kashiwagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetoshi","family":"Inoko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)92274-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)92186-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/143.4.509"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1986.tb02973.x"},{"key":"e_1_2_1_6_2","first-page":"638","volume":"68","author":"Uchiyama T.","year":"1987","journal-title":"Clin. 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