{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:17:13Z","timestamp":1770704233878,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":5740,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1990,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human interleukin 4 (IL 4) up\u2010regulates the expression of CD23 on both resting and \u201c<jats:italic>in vivo<\/jats:italic>\u201d activated B cells but induces proliferation and\/or differentiation only on \u201c<jats:italic>in vitro<\/jats:italic>\u201d activated B lymphocytes. Resting B cells express 360 high\u2010affinity IL 4 receptors (R) per cell (K<jats:sub>d<\/jats:sub> = 25\u201375 pM). Activation of resting B cells with anti\u2010IgM antibody or <jats:italic>Staphylococcus aureus<\/jats:italic> Cowan I (SAC) results in a two\u2010to\u2010threefold increase of IL 4R number without alteration of IL 4R affinity for IL 4. Flow cytometric analysis of biotinylated IL 4 binding shows that IL 4R expression is up\u2010regulated on virtually all anti\u2010IgM\u2010stimulated B cells, but only on a subpopulation of larger cells among SAC\u2010activated B lymphocytes. Culturing cells for 40 h with optimal concentrations of IL 4 does not significantly affect IL 4R levels on resting and anti\u2010IgM\u2010preactivated B lymphocytes but triples IL 4R levels on SAC\u2010preactivated B cells. Removal of IL 4 from cell cultures results in a two\u2010to\u2010fourfold increase of IL 4R levels 2 h later, suggesting an increase in IL 4R turnover. Resting and activated B cells degrade <jats:sup>125<\/jats:sup>1\u2010labeled IL 4 at 37 \u00b0C. Sodium dodecyl sulfate\u2010polyacrylamide gel electrophoretic analysis of IL 4 binding molecules on resting, \u201c<jats:italic>in vivo<\/jats:italic>\u201d activated and anti\u2010IgM\u2010activated B cells reveals the same three species of 130, 80\u201075, 70\u201065 kDa. Thus, IL 4 displays its different biological activities on resting and activated B cells through IL 4R of the same affinity, gross biochemical structure and ability to mediate IL 4 degradation.<\/jats:p>","DOI":"10.1002\/eji.1830200314","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T11:47:25Z","timestamp":1172749645000},"page":"551-555","source":"Crossref","is-referenced-by-count":30,"title":["Interleukin 4 receptors on normal human B lymphocytes: Characterization and regulation"],"prefix":"10.1002","volume":"20","author":[{"given":"Caroline E.","family":"Zuber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Pierre","family":"Galizzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Vall\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Howard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen","family":"Howard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Banchereau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.16.5894"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.05.040187.002241"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00744.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.6.1459"},{"key":"e_1_2_1_6_2","first-page":"224","volume":"66","author":"Shields J. J.","year":"1989","journal-title":"Immunology"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830190212"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.4049\/jimmunol.139.4.1135","volume":"139","author":"Defrance T.","year":"1987","journal-title":"J. Immunol."},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.4049\/jimmunol.141.6.2000","volume":"141","author":"Defrance T.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"10817","DOI":"10.1016\/S0021-9258(18)38043-8","volume":"263","author":"Le H. V.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90507-9"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.2.476"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"6984","DOI":"10.1016\/S0021-9258(18)83528-1","volume":"264","author":"Galizzi J. P.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.4049\/jimmunol.141.6.1982","volume":"141","author":"Galizzi J. P.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"456","DOI":"10.4049\/jimmunol.140.2.456","volume":"140","author":"Lowenthal J. W.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/325537a0"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.6.1669"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113589"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1987.tb00499.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.5.2214"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200122"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.8221"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830200314","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830200314","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T20:41:34Z","timestamp":1698007294000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830200314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,3]]}},"alternative-id":["10.1002\/eji.1830200314"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830200314","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3]]}}}