{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:50:33Z","timestamp":1771955433832,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":5556,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The staphylococcal exotoxin toxic shock syndrome toxin\u20101 (TSST\u20101) has potent stimulatory effects on murine and human lymphocytes. This is the consequence of TSST\u20101 binding to major histocompatibility complex (MHC) class II molecules and the engagement in a V<jats:sub>\u03b2<\/jats:sub>\u2010restricted fashion of the T cell receptor by the TSST\u20101\u2010MHC class II complex. Using radioligand and functional assays we have recently shown that TSST\u20101 binds to all HLA\u2010DR (<jats:italic>n<\/jats:italic> = 14), HLA\u2010DQ (<jats:italic>n<\/jats:italic> = 2) and HLA\u2010DP (<jats:italic>n<\/jats:italic> = 2) phenotypes tested. In this study, we have examined the ability of murine MHC class II molecules to bind TSST\u20101. Specific high\u2010affinity binding of TSST\u20101 was detectable to unfractionated BALB\u2010c (H\u20102<jats:sup>d<\/jats:sup>) and C57BL\/6 (H\u20102<jats:sup>b<\/jats:sup>), but not to C3H (H\u20102<jats:sup>k<\/jats:sup>) spleen cells. The K<jats:sub>d<\/jats:sub> of this binding estimated from Scatchard analysis was in the same nanomolar range as the K<jats:sub>d<\/jats:sub> of binding of TSST\u20101 to HLA\u2010DR. Binding of <jats:sup>125<\/jats:sup>I\u2010labeled TSST\u20101 to BALB\/c\u2010derived B cell lymphoma lines and to L cell transfectants correlated with the expression of I\u2010A molecules, but not with the expression of I\u2010E molecules. Furthermore, I\u2010A<jats:sup>+<\/jats:sup>, I\u2010E<jats:sup>\u2212<\/jats:sup> cells but not I\u2010A<jats:sup>\u2212<\/jats:sup>, I\u2010E<jats:sup>+<\/jats:sup> cells were able to support TSST\u20101\u2010induced T cell proliferation. The binding affinity of TSST\u20101 for I\u2010A<jats:sup>k<\/jats:sup> appears to be much lower than for I\u2010A<jats:sup>d<\/jats:sup>. L cell transfectants expressing hybrid DR\u03b1: I\u2010E<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tex2gif-stack-1.gif\" xlink:title=\"urn:x-wiley:00142980:media:EJI1830200907:tex2gif-stack-1\"\/> molecules, but not those expressing I\u2010E<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tex2gif-stack-2.gif\" xlink:title=\"urn:x-wiley:00142980:media:EJI1830200907:tex2gif-stack-2\"\/>: DR1<jats:sub>\u03b2<\/jats:sub> molecules, could bind TSST\u20101 and efficiently support TSST\u20101\u2010induced T cell proliferation. This suggests that minor differences in the highly homologous I\u2010E<jats:sub>\u03b1<\/jats:sub> and DR<jats:sub>\u03b1<\/jats:sub> chains are critical in determining the affinity of the MHC class II molecule for TSST\u20101. These results demonstrate that the binding of TSST\u20101 to MHC class II molecules in the mouse, in contrast to humans, is strongly influenced by phenotype. Analysis of the molecular basis of these differences may help to localize staphylococcal exotoxin binding sites on MHC class II molecules.<\/jats:p>","DOI":"10.1002\/eji.1830200907","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T03:25:28Z","timestamp":1172719528000},"page":"1911-1916","source":"Crossref","is-referenced-by-count":24,"title":["Binding of toxic shock syndrome toxin\u20101 to murine major histocompatibility complex class II molecules"],"prefix":"10.1002","volume":"20","author":[{"given":"Paul R.","family":"Scholl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafick\u2010P.","family":"Sekaly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Diez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie H.","family":"Glimcher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raif S.","family":"Geha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198012183032501"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)92186-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/143.4.509"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1128\/iai.55.5.1070-1076.1987","volume":"55","author":"Parsonnet J.","year":"1987","journal-title":"Infect. Immun."},{"key":"e_1_2_1_6_2","first-page":"247","volume-title":"The Staphylococci","author":"Bergdoll M. S.","year":"1985"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/151.1.65"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.6.2011"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111334"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/151.3.514"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/158.5.1026"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.4049\/jimmunol.142.8.2855","volume":"142","author":"Galelli A.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.5.1697"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1989.tb00003.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.4049\/jimmunol.142.9.3151","volume":"142","author":"Fischer H.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1128\/iai.57.6.1816-1824.1989","volume":"57","author":"Vroegop S. M.","year":"1989","journal-title":"Infect. Immun."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/339221a0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2658055"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.11.4210"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.4049\/jimmunol.143.8.2583","volume":"143","author":"Scholl P. R.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"226","DOI":"10.4049\/jimmunol.144.1.226","volume":"144","author":"Scholl P. R.","year":"1990","journal-title":"J. Immunol."},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90980-X"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2524876"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00350120"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.4049\/jimmunol.141.6.2158","volume":"141","author":"Klohe E. P.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90225-3"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.17.5885"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.2.541"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(87)90068-7"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(89)90177-9"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.1988.tb00417.x"},{"key":"e_1_2_1_32_2","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1128\/iai.33.3.827-833.1981","volume":"33","author":"Buxser S.","year":"1981","journal-title":"Infect. Immun."},{"key":"e_1_2_1_33_2","doi-asserted-by":"crossref","first-page":"3175","DOI":"10.4049\/jimmunol.143.10.3175","volume":"143","author":"Uchiyama T.","year":"1989","journal-title":"J. Immunol."}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830200907","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830200907","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T20:33:04Z","timestamp":1698006784000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830200907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9]]},"references-count":32,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1002\/eji.1830200907"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830200907","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9]]}}}