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We have used polymerase chain reaction gene amplification in conjunction with a panel of V<jats:sub>H<\/jats:sub> family\u2010specific amplimers to directly compare the repertoire of V<jats:sub>H<\/jats:sub> region rearrangement in mature, CD5<jats:sup>+<\/jats:sup> B cell chronic lymphocytic leukemia with that in immature, CD5 B lineage acute lymphoblastic leukemia. The results revealed a diverse pattern of V<jats:sub>H<\/jats:sub> family utilization common to both disease groups in which V<jats:sub>H<\/jats:sub> regions most proximal to the IgH joining locus were preferentially rearranged relative to their family sizes with recurrent utilization of several known developmentally restricted V<jats:sub>H<\/jats:sub> genes in close to germ\u2010line configuration. These results indicate that biased V<jats:sub>H<\/jats:sub> family usage is independent of tumor cell phenotype in B lineage leukemias. This bias may reflect similar stages or compartments in normal B lymphopoiesis from which diverse types of B cell malignancy may arise. Moreover, since blast cells in acute lymphoblastic leukaemia do not express functional immunoglobulin, we infer that the tumor cell\u2010associated V<jats:sub>H<\/jats:sub> family repertoire is determined through antigen\u2010independent mechanisms.<\/jats:p>","DOI":"10.1002\/eji.1830201009","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T11:34:39Z","timestamp":1172748879000},"page":"2209-2217","source":"Crossref","is-referenced-by-count":112,"title":["Immunoglobulin heavy chain variable region family usage is independent of tumor cell phenotype in human B lineage leukemias"],"prefix":"10.1002","volume":"20","author":[{"given":"Miriam","family":"Deane","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Norton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1986.tb01470.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90400-1"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02912.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1002\/j.1460-2075.1988.tb03039.x","volume":"7","author":"Buluwela L.","year":"1988","journal-title":"EMBO J."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03309.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.3.1267"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/331446a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.8196"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-71851-9_6"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(86)90239-1"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"706","DOI":"10.4049\/jimmunol.143.2.706","volume":"146","author":"Souroujon M. 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