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We have previously shown that the stimulatory capacity of the tumor cells is increased after EBV conversion (Avila\u2010Carino <jats:italic>et al., Int. J. Cancer<\/jats:italic> 1987. <jats:italic>40<\/jats:italic>: 691). As a first step towards the identification of the viral gene product responsible for this change we have studied the influence of the EBV latent membrane protein (LMP) on the stimulatory capacity of the EBV\u2010negative BL lines BL41 and DG75 and the B lymphoma line BJAB.<\/jats:p><jats:p>Four LMP\u2010transfected sublines of BL41, four DG75 LMP transfectants and one LMP\u2010transfected subline of BJAB showed a significantly stronger stimulatory capacity than the original line. The effect was directly proportional to the amount of LMP detected in each transfectant but was not due to reactivation of LMP\u2010specific memory cells since lymphocytes from EBV\u2010seropositive and \u2010seronegative individuals responded equally. In order to define the relation between LMP expression and induction of stimulatory capacity, DG75 was transfected with constructs containing the LMP gene under the control of an heat\u2010shock promoter. The peak of LMP expression in heat shock\u2010treated cells preceded the appearance of stimulatory capacity by 6\u201312 h suggesting that critical amounts of the protein may be required to induce the phenotypic change recognized by the T cells.<\/jats:p><jats:p>LMP influenced in a dose\u2010dependent manner the expression of the adhesion molecules LFA\u20101, LFA\u20103 and ICAM\u20101 and B cell activation markers CD23 and CD39 in transfected sublines of BL41, but did not affect the expression of these markers in the DG75 and BJAB cell line. All LMP\u2010expressing transfectants showed an increased capacity to form conjugates with unprimed allogeneic lymphocytes.<\/jats:p>","DOI":"10.1002\/eji.1830201019","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T11:34:39Z","timestamp":1172748879000},"page":"2293-2299","source":"Crossref","is-referenced-by-count":32,"title":["Expression of the Epstein\u2010Barr virus (EBV)\u2010encoded membrane antigen (LMP) increases the stimulatory capacity of EBV\u2010negative B lymphoma lines in allogeneic mixed lymphocyte cultures"],"prefix":"10.1002","volume":"20","author":[{"given":"Laura","family":"Cuomo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankaj","family":"Trivedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6sta","family":"Winberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Masucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60436-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910150217"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/267052a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/294458a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910290403"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910380414"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.137.4.1270"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(75)92004-8"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.4049\/jimmunol.134.4.2287","volume":"134","author":"Konttinen Y. 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