{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T10:45:37Z","timestamp":1698144337957},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":5380,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In B chronic lymphocytic leukemia (B\u2010CLL) cells, lipopolysaccharide (LPS) and phorbol esters fail to activate the plasma membrane\u2010associated Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> anti\u2010porter and, subsequently, to elicit a rise in cytosolic pH. Since these events are thought to be a prerequisite for LPS\u2010induced proliferation of B normal lymphocytes, we analyzed the kinetic properties of Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> antiporter in B\u2010CLL cells as compared to both CD5<jats:sup>\u2212<\/jats:sup> and CD5<jats:sup>+<\/jats:sup> normal B lymphocytes. In the present work we report that Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchange rate after acid loading is drastically decreased in B\u2010CLL cells, as compared to normal CD5<jats:sup>\u2212<\/jats:sup> B lymphocytes, although the antiporter affinity for external Na<jats:sup>+<\/jats:sup> and internal H<jats:sup>+<\/jats:sup> is not significantly different in both cell populations. Kinetic data account for a reduction in the number of operating antiport units in B\u2010CLL. The Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> antiporter of CD5<jats:sup>+<\/jats:sup> normal B lymphocytes exhibits both an exchange rate and an ion affinity significantly higher than that observed in both CD5<jats:sup>+<\/jats:sup> B\u2010CLL cells and CD5<jats:sup>\u2212<\/jats:sup> B normal lymphocytes, thus suggesting a possible explanation for their activated phenotype.<\/jats:p>","DOI":"10.1002\/eji.1830210308","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T12:39:06Z","timestamp":1172752746000},"page":"583-588","source":"Crossref","is-referenced-by-count":10,"title":["Na<sup>+<\/sup>\/H<sup>+<\/sup> antiporter has different properties in human B lymphocytes according to CD5 expression and malignant phenotype"],"prefix":"10.1002","volume":"21","author":[{"given":"Gianluca","family":"Gaidano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Caligaris\u2010Cappio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Treves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Bussolino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianpiero","family":"Pescarmona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dario","family":"Ghigo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amalia","family":"Bosia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90901-X"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2154036"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869680"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112671"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.48.030186.002051"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(89)90004-X"},{"key":"e_1_2_1_8_2","first-page":"205","volume":"49","author":"Rotin D.","year":"1989","journal-title":"Cancer Res."},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"913","DOI":"10.4049\/jimmunol.142.3.913","volume":"142","author":"Gaidano G.","year":"1989","journal-title":"J. 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